Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Studies on the Biological Activity of Rosemarinus officinalis L.

Rosemary (Rosemarinus officinalis L.) 추출물의 생리활성 탐색

  • Cho, Young-Je (Department of Food Engineering, Sangju National University) ;
  • Kim, Jeung-Hoan (Department of Food Engineering, Sangju National University) ;
  • Yoon, So-Jung (Department of Food Engineering, Sangju National University) ;
  • Chun, Sung-Sook (Department of Food Science & Technology, Yeungnam University) ;
  • Choi, Ung-Kyu (Department of Oriental Medical Food & Nutrition, Asia University)
  • 조영제 (상주대학교 식품공학과) ;
  • 김정환 (상주대학교 식품공학과) ;
  • 윤소정 (상주대학교 식품공학과) ;
  • 천성숙 (영남대학교 식품가공학과) ;
  • 최웅규 (아시아대학교 한방식품영양학과)
  • Published : 2005.12.31
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Abstract

Based on their biological activity, phenols from rosemary extract were evaluated for inhibition of Helicobacter pylori. Contents of total phenolic compounds and inhibition zone of water and ethanol extracts from rosemary were 24.3mg/g and 25.7mg/g, and 11mm, 14mm, and, at $200{\mu}g/mL$ phenol content, 20.9% and 78.2% inhibitory activities were observed, respectively. Electron donating abilities and 2.2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid cation radicals of water and ethanol extracts were 89.1% and 62.0% and 98.4% and 96.5%, respectively. Thiobarbituric acid reactive substances values of all extracts were lower than that of control. Ethanol extract showed 98.8% angiotensin-converting enzyme inhibitory activity. Xanthin oxidase inhibitory activities of water and ethanol extracts were very high, at 84.8%, 100%, respectively. These results indicated phenolic compounds from rosemary can be utilize as a potential antioxidant, antimicrobial, anti-hypertension and anti-gout sources.

본 연구에서는 건조된 rosemary잎을 물과 60% 에탄올로 추출하여 H. pylori항균활성과 항산화 및 기타 생리활성 효과를 알아보았다. Rosemary 추출물의 총 페놀 함량은 열수추출물에서 24.3mg/g였으며 알코올 추출물에서는 25.7mg/g으로 알코올추출물에 더 높은 페놀함량을 나타내었다. H. pylori에 대한 항균활성은 고체 배지상에서 $200{\mu}g/mL$의 페놀함량을 첨가했을 때 저해환의 직경은 물 추출물에서 11mm, 알코올 추출물에서 14mm로 나타났으며 액체 배지상에서는 각각 20.9, 78.2%의 저해율로 나타났다. 항산화활성은 물추출물과 60% 에탄올 추출물에서 각각 전자공여능이 89.1, 62.0%, $ABTS^+$는 98.42, 96.5%, PF는 0.9, 1.2, TBARS값은 대조구에$(2.4{\times}10^{-3}{\mu}M)$비해 알코올 추출물$(0.3{\times}10^{-3}{\mu}M)$과 물 추출물$(0.21{\times}10^{-3}{\mu}M)$이 더 낮은 TBARS값을 나타내었다. Angiotensin converting enzyme과 xanthin Oxidase 저해율은 열수 추출물에서 각각 39.9, 84.8% 그리고 알코올 추출물에서 89.8, 100%의 결과로 나타났다. 이 결과로 보아 로즈마리 추출물 중의 페놀성 화합물은 항산화활성, 항균활성, 고혈압 및 통풍에 효과가 있을 것으로 사료된다.

Keywords

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