한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Docosahexaenoic acid 함유 재구성지질의 효소적 합성 연구

Lipase-Catalyzed Synthesis of DHA-Enriched Structured Lipid

  • Cho, Eun-Jin (Department of Food Science and Technology, Chungnam National University) ;
  • Cho, Kyung-Hyun (School of Biotechnology, Yeungnam University) ;
  • Lee, Ki-Taek (Department of Food Science and Technology, Chungnam National University)
  • 발행 : 2005.10.31
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초록

조류(microalgae, from Schizochytrium sp.)로부터 DHA가 풍부하고 이취가 적은 지질을 획득하고 이를 옥수수유와 함께 반응기질로 이용하여 재구성지질을 합성하였다. 본 실험에 사용된 합성조건으로 oleic(17.3 mol%), linoleic(31.8 mol%), 그리고 docosahexaenoic acid(14.9 mol%)가 함유된 재구성지질을 합성하였다. ${\alpha}-,\;{\gamma}-$, 및 ${\delta}-tocopherol$의 총 함량은 0.059%로 분석되었고, 비누화 가(183.8) 및 요오드 가(201.8)를 측정하였다. 한편, 수행된 반응조건 및 반응시간동안 대부분의 반응은 6시간 내에 이루어 졌고, 그 상승폭은 3-6시간 사이에서 약 2.3배의 증가를 보이며 가장 크게 나타났다.

Structured lipid (SL) was synthesized by enzymatic interesterification of algae oil and corn oil in stirred tank batch reactor, The reaction, performed for 15hr at $65^{\circ}C$, was catalyzed by sn-1,3-specific lipase RM IM from Rhizonucor miehei without organic solvent. DHA, oleic acid, and linoleic acid contents of SL were 14.9, 17.3, and 31.8 mol%, respectively. ${\alpha}-,\;{\gamma}-,\;and\;{\delta}-tocopherol$ contents and physiochemical property of SL were evaluated. During 15 hr reaction, most reaction occurred within 6 hr, and highest relative production rate was observed between 3 to 6 hr.

키워드

참고문헌

  1. Sardesai VM. Nutritional role of polyunsaturated fatty acids. J. Nutr. Biochem. 3: 154-166 (1992) https://doi.org/10.1016/0955-2863(92)90110-5
  2. Simopoulos AP. Omega-3 fatty acids in health and disease and in growth and development. Am. J. Clin. Nutr. 54: 438-463 (1991) https://doi.org/10.1093/ajcn/54.3.438
  3. Horrocks LA, Yeo YK. Health benefits of docosahexaenoic acid(DHA). Pharm. Res. 40: 211-225 (1999) https://doi.org/10.1006/phrs.1999.0495
  4. Tonon T, Harvey D, Larson TR, Graham IA. Long chain polyunsaturated fatty acid production and partitioning to triacylglycerol in four microalgae. Phytochemistry 61: 15-24 (2002) https://doi.org/10.1016/S0031-9422(02)00201-7
  5. Hammond BG, Mayhew DA, Naylor MW, Ruecker FA, Mast RW, Sander WJ, Safety assessment of DHA-rich micoalgae from Schizochytrium sp., I. Subchronic rat feeding study. Regul. Toxicol. Pharmacol. 33: 192-204 (2001) https://doi.org/10.1006/rtph.2001.1458
  6. Abril R, Garrett J, Zeller So, Sander WJ, Mast RW. Safety assessment of DHA-rich micoalgae from Schizochytrium sp., V. Target animal safety/toxicity study in growing swine. Regul. Toxicol. Pharmacol. 37: 73-82 (2003) https://doi.org/10.1016/S0273-2300(02)00030-2
  7. Cho EJ, Lee JH, Lee KT. Optimization of enzymatic condition of structured lipids by response surface methodology. Korean J. Food Sci. Technol. 36: 531-536 (2004)
  8. Lee KT, Akoh CC. Structured lipids: synthesis and applications. Food Rev. Int.14: 17-34(1998) https://doi.org/10.1080/87559129809541148
  9. Akoh CC. Structured lipid: enzymatic approach. Inform 6: 1055-1061 (1995)
  10. Small DM. The effects of glyceride structure on absorption and metabolism. Ann. Res. Nutr. 11: 413-419 (1991) https://doi.org/10.1146/annurev.nu.11.070191.002213
  11. Lee KT, Akoh CC. Immobilized lipase-catalyzed production of structured lipids with eicosapentaenoic acid at specific position. J. Am. Oil Chem. Soc. 73: 611-615 (1996) https://doi.org/10.1007/BF02518116
  12. Cho EJ, Lee KT. Synthesis of structured lipids from com oil and conjugated linoleic acid with immobilized lipase-catalyzed reaction. Korean J. Food Sci. Technol. 35: 797-802 (2003)
  13. AOCS. Official Methods and Recommended Practices of the American Oil Chemists' Society. 4th ed. Cd 3a-63. American Oil Chemists' Society, Champaign, IL, USA (1990)
  14. AOCS. Official Methods and Recommended Practices of the American Oil Chemists' Society. 4th ed. Cd 3-25. American Oil Chemists' Society, Champaign, Champaign IL, USA (1990)
  15. AOCS. Official Methods and Recommended Practices of the American Oil Chemists' Society. 4th ed. Cd 1-25. American Oil Chemists' Society, Champaign, IL, USA (1990)