Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Lipase-Catalyzed Synthesis of DHA-Enriched Structured Lipid

Docosahexaenoic acid 함유 재구성지질의 효소적 합성 연구

  • Cho, Eun-Jin (Department of Food Science and Technology, Chungnam National University) ;
  • Cho, Kyung-Hyun (School of Biotechnology, Yeungnam University) ;
  • Lee, Ki-Taek (Department of Food Science and Technology, Chungnam National University)
  • Published : 2005.10.31
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Abstract

Structured lipid (SL) was synthesized by enzymatic interesterification of algae oil and corn oil in stirred tank batch reactor, The reaction, performed for 15hr at $65^{\circ}C$, was catalyzed by sn-1,3-specific lipase RM IM from Rhizonucor miehei without organic solvent. DHA, oleic acid, and linoleic acid contents of SL were 14.9, 17.3, and 31.8 mol%, respectively. ${\alpha}-,\;{\gamma}-,\;and\;{\delta}-tocopherol$ contents and physiochemical property of SL were evaluated. During 15 hr reaction, most reaction occurred within 6 hr, and highest relative production rate was observed between 3 to 6 hr.

조류(microalgae, from Schizochytrium sp.)로부터 DHA가 풍부하고 이취가 적은 지질을 획득하고 이를 옥수수유와 함께 반응기질로 이용하여 재구성지질을 합성하였다. 본 실험에 사용된 합성조건으로 oleic(17.3 mol%), linoleic(31.8 mol%), 그리고 docosahexaenoic acid(14.9 mol%)가 함유된 재구성지질을 합성하였다. ${\alpha}-,\;{\gamma}-$, 및 ${\delta}-tocopherol$의 총 함량은 0.059%로 분석되었고, 비누화 가(183.8) 및 요오드 가(201.8)를 측정하였다. 한편, 수행된 반응조건 및 반응시간동안 대부분의 반응은 6시간 내에 이루어 졌고, 그 상승폭은 3-6시간 사이에서 약 2.3배의 증가를 보이며 가장 크게 나타났다.

Keywords

References

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