Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Effect of Korean folk medicine 'SecSec' on inflammatory cytokine secretion in HMC-1 cells

  • Choi, In-Young (College of Oriental Medicine, Kyung Hee University,College of Pharmacy, Wonkwang University) ;
  • Kim, Mi-Sun (College of Oriental Medicine, Kyung Hee University) ;
  • Koo, Hyoun-Na (College of Oriental Medicine, Kyung Hee University) ;
  • Hong, Seung-Hun (College of Pharmacy, Wonkwang University) ;
  • Kim, Hyung-Min (College of Oriental Medicine, Kyung Hee University) ;
  • Um, Jae-Young (College of Oriental Medicine, Kyung Hee University)
  • Published : 2005.03.30
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Abstract

'SecSec' has been used for the purpose of prevention and treatment of throat diseases such as sore throat, cough, bronchial asthma and allergic asthma in Korea. However, its effect in experimental models remains unknown. To investigate the biological effect of SecSec, we examined cytotoxicity and secretion of inflammatory cytokines on human leukemic mast cell line, HMC-1, stimulated with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. SecSec by itself had no cytotoxicity on HMC-1. When SecSec (1 mg/ml) was added, the secretion of tumor necrosis factor-alpha $(TNF-{\alpha})$, interleukin (IL)-6, and granulocyte macrophage-colony stimulating factor (GM-CSF) was significantly inhibited about 47.20%, 25.55%, and 46.43%, respectively on PMA plus A23187-stimulated HMC-1 cells. But SecSec did not inhibit IL-8 secretion. These findings may help understanding the mechanism of action of this medicine leading to control activated mast cells on allergic inflammatory condition like asthma.

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References

  1. Babu KS,Davies DE, Holgate ST, (2004) Role of tumor necrosis factor alpha in asthma. Immunol. Allergy Clin. North Am. 24(4),583-597,v-vi. https://doi.org/10.1016/j.iac.2004.06.010
  2. Barnes PJ. (1995) Inhaled glucocorticoids for asthma. N. Engl. J. Med. 332, 868-875. https://doi.org/10.1056/NEJM199503303321307
  3. Barnes PJI. (2000) Chronic obstructive pulmonary disease. N. Engl. J. Med. 343, 269-280. https://doi.org/10.1056/NEJM200007273430407
  4. Barnes PJ. (2004) New drugs for asthma. Nat. Rev. Drug Discov. 3(10), 831-844. https://doi.org/10.1038/nrd1524
  5. Bittleman DB, Casale TB. (1994) Allergic models and cytokines. Am. J. Respir. Crit. Care Med. 150, s72-76.
  6. Bradding P, Holgate ST, (1999) Immunopathology and human mast cell cytokines. Critical Reviews in Oncology: Hematology 31, 119-133.
  7. Broide DH, Lotz M, Cuomo AJ, Coburn DA, Federman EC, Wasserman SI. (1992) Cytokines in symptomatic asthma airways. J. Allergy Clin. lmmunol. 89,958-967. https://doi.org/10.1016/0091-6749(92)90218-Q
  8. Cromwell O, Hamid Q, Corrigan CJ, Barkans J, Meng Q, Collins PD, Kay AB. (1992) Expression and generation of interleukin-8, IL-6 and granulocyte-macrophage colony-stimulating factor by bronchial epithelial cells and enhancement by IL-1 beta and tumour necrosis factor-alpha. Immunology 77,330-337.
  9. Gajewska BU, Wiley RE, Jordana M. (2003) GM-CSF and dendritic cells in allergic airway inflammation: basic mechanisms and prospects for therapeutic intervention. Curr. Drug Targets Inflamm. Allergy 2, 279-292. https://doi.org/10.2174/1568010033484052
  10. Gasson JC (1991) Molecular physiology of granulocytemacrophage colony-stimulating factor. Blood 77, 1131-1145.
  11. Gosset P, Tsicopoulos A, Wallaert B, Vannimenus C. Joseph M, Tonnel AB, Capron A. (1991) Increased secretion by tumor necrosis factor-a and interleukin 6 by alveolar macrophages consecutive to the development of the late asthmatic reaction. J. Allergy Clin. lmmunol. 88,561-571. https://doi.org/10.1016/0091-6749(91)90149-I
  12. Hamid Q, Tulic' MK, Liu Me, Moqbel R. (2003) Inflammatory cells in asthma: mechanisms and implications for therapy. J. Allergy Clin. Immunol. 111 (1 Suppl), S5-S17. https://doi.org/10.1067/mai.2003.22
  13. Hosoda M, Yamaya M, Suzuki T, Yamada N, Kamanaka M,Sekizawa K,Butterfield JH, Watanabe T, Nishimura H, Sasaki H. (2002) Effects of rhinovirus infection on histamine and cytokine secretion by cell lines from human mast cells and basophils. J. Immunol. 169 (3),1482-1491. https://doi.org/10.4049/jimmunol.169.3.1482
  14. Jagels MA, Daffern PJ, Zuraw BL, Hugli TE. (1991) Mechanisms and regulation of polymorphonuclear leukocyte and eosinophil adherence to human airway epithelial cells. Am. J. Respir. Cell Mol. BioI. 21, 418-427.
  15. Kim MS, Na HJ, Han SW, Jin JS, Song UY, Lee EJ, Song BK, Hong SH, Kim HM. (2003) Forsythia fructus inhibits the mast-ceIl-mediated allergic inflammatory reactions. Inflammation 27(3),129-135. https://doi.org/10.1023/A:1023865727780
  16. Kips JC. Tavernier J, Pauwels RA. (1992) Tumor necrosis factor causes bronchial hyperresponsiveness in rats. Am. Rev. Respir. Dis. 145, 332-336. https://doi.org/10.1164/ajrccm/145.2_Pt_1.332
  17. Kwon O, Au BT, Collins PD, Mak JC, Robbins RR, Chung KF, Barnes PJ. (1994) Tumour necrosis factor-induced interleukin-8 expression in pulmonary cultured human airway epithelial cells. Am. J. Physiol. 267, L398-L405.
  18. Lei XF, Ohkawara Y, Stampfli MR, Gauldie J, Croitoru K, Jordana M, Xing Z. (1998) Compartmentalized transgene expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in mouse lung enhances allergic airways inflammation. Clin. Exp. Immunol. 113, 157-165. https://doi.org/10.1046/j.1365-2249.1998.00652.x
  19. Masuda T, Suda Y, Shimura S, Maruyama N, Aizawa T, Tamura G, Sasaki H, Takishima T. (1992)Airway epithelial cellsenhance eosinophil survival. Respiration 59,438-422.
  20. Oh JW, Lee HB, Kim CR, Yum MK, Koh YJ, Moon SI, Kang JO, Park IL. (1999) Analysis of induced sputum to examine the effects of inhaled corticosteroid on airway inflammation in children with asthma. Ann. Allergy Asthma Immunol. 82, 491-496. https://doi.org/10.1016/S1081-1206(10)62727-5
  21. Shah A, Church MK, Holgate ST. (1995) Tumour necrosis factor-a: A potential mediator of asthma. Clin. Exp. Allergy 25, 1038-1044. https://doi.org/10.1111/j.1365-2222.1995.tb03249.x
  22. Stankiewicz W, Dabrowski MP, Chcialowski A, Plusa T. (2002) Cellular and cytokine immunoregulation in patients with chronic obstructive pulmonary disease and bronchial asthma. Mediat. Inflamm. 11(5),307-312. https://doi.org/10.1080/09629350210000015719
  23. Thomas PS, Yates DH, Bames PJ. (1995) Tumor necrosis factor-alpha increases airway responsiveness and sputum neutrophilia in normal human subjects. Am. J. Respir. Crit. Care Med. 152(1), 76-80. https://doi.org/10.1164/ajrccm.152.1.7599866
  24. Vignola AM, Chanez P, Chiappara G, Siena L, Merendino A, Reina C, Gagliardo R, Profita M, Bousquet J, Bonsignore G. (1999) Evaluation of apoptosis of eosinophils, macrophages, and T lymphocytes in mucosal biopsy specimens of patients with asthma and chronic bronchitis. J. AllergyClin. Immunol. 103, 563-573. https://doi.org/10.1016/S0091-6749(99)70225-3
  25. Yamashita N, Tashimo H, Ishida H, Kaneko F, Nakano J, Kato H, Hirai K, Horiuchi T, Ohta K (2002) Attenuation of airway hyperresponsiveness in a murine asthma model by neutralization of granulocyte-macrophage colony-stimulating factor (GM-CSF). Cell Immunol. 219, 92-97. https://doi.org/10.1016/S0008-8749(02)00565-8
  26. Yousefi SK, Blaser H, Simon U. (1997) Activation of signaling pathways and prevention of apoptosis by cytokines in eosinophils. Int. Arch.Allergy Immunol. 112, 9-12. https://doi.org/10.1159/000237424