The Anti-inflammatory and Analgesic Activities of Artemisia capillaris Thunberg

인진 추출물의 소염진통작용

  • 김시나 ((주)KMSI 부설 한국의과학연구소) ;
  • 김희석 ((주)KMSI 부설 한국의과학연구소) ;
  • 남경숙 ((주)KMSI 부설 한국의과학연구소) ;
  • 황성완 ((주)KMSI 부설 한국의과학연구소) ;
  • 황성연 ((주)KMSI 부설 한국의과학연구소)
  • Published : 2005.12.30

Abstract

Inflammatory mediators such as interleukin-1 (IL-1), tumor necrosis $factor-{\alpha}\;(TNF-{\alpha}),\;interferon-{\gamma}\;(IFN-{\gamma})$ and lipopolysaccharide (LPS) are thought to play major roles in joint diseases such as a rheumatoid arthritis (RA), and there is considerable evidence playing a role for these cytokines in osteoarthritis (OA). Therefore, we have studied the effects on anti-inflammation and analgesic by ethyl acetate fraction from 70% ethanol extract of Artemisia capillaries (EAC). As a positive control, apigenin, which is known as an anti-inflammatory agent as an iNOS inhibitor, was used and showed the dose-dependent inhibitory effect. EAC showed strong inhibitory efficacy against cytokine-induced proteoglycan degradation, $PGE_2$ production, nitric oxide (NO) production, and matrix-matalloproteinases (MMPs) expression in rabbit articular chondrocyte. In the writhing test induced by acetic acid, EAC $(200{\sim}400\;mg/kg)$ exhibited a dose-dependent inhibition of writhing. The results indicate that EAC have anti-inflammatory and analgesic activities, and could be a good herbal medicine candidate for curing of RA and/or OA.

Keywords

References

  1. Nam, K. W., Je, K H., Lee, J. H., Han, H. J., Lee, H. J., Kang, S. K and Mar, W. C. (2003) Inhibition of COX-2 activity and proinflammatory cytokines (TNF-$\alpha$ and IL-1$\beta$) Production by water-soluble sub-fractionated parts from Bee (Apis mellifera) Venom. Arch. Pharm. Res. 26: 383-388 https://doi.org/10.1007/BF02976695
  2. Moon, Y H., Go, J. J. and Park, J. Y (1999) The anti-inflammatory and analgesic activities of Gumiganghwaltang. Kor. J Pharmacogn. 30: 18-24
  3. Ha, H. K, Lee, J. H. and Kim, C. S. (2000) Extracts of Rheum undulatum L. Inhibitis COX-2 activities in lipopolysaccharide-stimulated Raw 264.7 cells. J Applied. Pharmacol. 8: 73-77
  4. Badger, A. M., Cook, M. N., Swift, B.A., Newmantraa, T. M., Gowen, M. and Lark, M. (1999) Inhibition of Interleukin- 1 induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, Hymenialdisine. J. Pharmacol. Exp. Ther. 290: 587-593
  5. Gowen, M., Wood, D.D., Ihrie, E.J. and Russel, R.G.G. (1984) Stimulation by human IL-1 of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblasts in vitro. Biochem. Biophys. Acta. 797: 186-193
  6. Westacott, C. I. and Sharif, M. (1996) Cytokines in osteoarthritis: Mediators or markers of joint destruction. Semin Arthritis Rheum. 25: 254-272 https://doi.org/10.1016/S0049-0172(96)80036-9
  7. Lee, S. H., Seo, G.S., Sohn, D.H. (2004) Inhibition of lipopolysaccharided-induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochem. Biophys.Res.Commun. 323:125-132 https://doi.org/10.1016/j.bbrc.2004.08.063
  8. Park, B. J., Cho, M. H., Kim, K H., Lee, S. K, Lee, C. S., An, G H. and Mar, W. C. (1999) Effect of natural products on the inhibition of Lipopolysacchrade-induced nitric oxide synthase activity in RAW 264.7 cell culture system. Nat. Prod. Sci. 5: 113-120
  9. Kim, H.T., Ahn, J.S., Jeong, I. H., et al.(1996) Subacute toxicity of SKl306X, an anti-inflammatory herbal extract, in rats. J. Applied. Pharmacol. 1: 19-31
  10. Sandy, J.D., Brown, H.L.G, Lowther, D.A. (1978) Degradation of proteoglycan in acticular cartilage. Biochem. Biophys. Acta. 543: 539-544
  11. Kim, J. Y, Park, S. W. (2004) Effect of Geraniol on the Proliferation of L1210 Cells and ICR Mouse Macrophages, and the Activities of Superoxide Dismutase (SOD) and Inducible Nitric Oxide Synthase (iNOS) Activities. Yahhak Hoeji 48: 309-316
  12. Elliott, S., Cawston, T. (2002) The clinical potential of matrix metalloproteinase inhibitors in rheumatic disorders. Drug Aging. 18: 87-99 https://doi.org/10.2165/00002512-200118020-00002
  13. Farrell, A. J., Blake, D. R., Palmer R, M. J. and Moncada. (1992) Increased concentrations of nitric synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases. Ann. Rheum. Dis. 51: 1219-1222 https://doi.org/10.1136/ard.51.11.1219
  14. Murrel, G.A.C., Jang, D. and Williams, R.J. (1995) Nitric oxide activates metalloprotease enzymes in articular cartilage. Biochem. Biophys. Res. Commun. 206: 15-21 https://doi.org/10.1006/bbrc.1995.1003
  15. Delafuente, J. C. (2000) Glucosamine in the treatment of osteoarthritis. Rheum. Dis. Clin. North. Am. 26: 1-11 https://doi.org/10.1016/S0889-857X(05)70116-2
  16. Chun, H. J., Ahn, B. Y, Han, J. H., and Woo, H. W (2001) Inhibitory effects of crude polysaccharide of water extract of Artemisia iwayomogi Kitamura on melanin biosynthesis. Yahhak Hoeji. 45: 701-707
  17. Lee, H. J., Hwang, E. H., Yu, H. H., Song, I. S., Kim, C. M., Kim, M. C., Hong, J. H., Kim, D. S., Han, S. B., Kang, K. J., Lee, E. J. and Chung, H. W (2002) The analysis ofnutrients in Artemisia capillsris Thunberg. J. Korean Soc. Food. Sci. Nutr. 31: 361-366 https://doi.org/10.3746/jkfn.2002.31.3.361
  18. Baek, T. W, Lee, K. A., Ahn, M. J., Joo, H. K., Cho, M. C., Kang, J. W, Kim, H. S. and Shim, J. H. (2004) Effects of 3,5-di-O-Caffeoylquinic acid from Artemisia scoparia Waldstein et Kitamura on the Function of HPV 16 Oncoproteins. Kor. J Pharmacogn. 35: 368-374