생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
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저산소/재관류로부터 청폐사간탕의 PC12 세포 보호 효과

Protective Effect of Metabolized Chungpesagan-tang on Hypoxia/Reperfusion Induced-PC12 Cell Damage

  • 소윤조 (전북대학교 치과대학 약리학교실)
  • Soh, Yun-Jo (Department of Pharmacology, School of Dentistry, Chonbuk National University)
  • 발행 : 2005.06.30
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초록

This research was performed to investigate the protective effect of Chungpesagan-tang (CST) from hypoxia/reperfusion induced-PC12 cell damage. To elucidate the mechanism of the protective effect of CST, cell viability, changes in activities of superoxide dismutase, glutathione peroxidase, catalase, caspase 3 and the production of malondialdehyde were observed after treating PC12 cells with CST which was metabolized by rat liver homogenate. Pretreatment of CST with liver homogenate appeared to increase its protective effect against hypoxia/reperfusion insult. The result showed that CST exhibited the highest protective effect against hypoxia/reperfusion at the dose of $1\;{\mu}g/ml$ in PC12 cells, probably by recovering the redox enzyme activities and MDA to control level.

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