Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Neuroprotective Effect of Acanthopanax sessiliflorus against Toxicity Induced by N-Methyl-D-Aspartate in Rat Organotypic Hippocampal Slice Culture

  • Lee, Pyeong-Jae (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Choi, Sang-Yoon (Korea Food Research Institute) ;
  • Son, Dong-Wook (KT&G Central Research Institute)
  • Published : 2005.09.01
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Abstract

We investigated that water extract of Acanthopanax sessiliflorus roots rescued the N-methyl-D-aspartate (NMDA), agonist of glutamate receptor, -induced toxicity in rat organotypic hippocampal slice culture. When the cell death in NMDA only-treated hippocampal slices was set 100%, A. sessiliflorus decreased the cell death to 75.4, 51.6, 48.9, and 40.6% at 1, 10, 50, and $100\;{\mu}g/ml$ treatment, respectively. On the basis of these results, the water extract of A. sessiliflorus roots may be a preventive agent against NMDA-induced cytotoxicity.

Keywords

References

  1. Andrius B. and Yana A., Neuroprotective effects of extracellular glutamate are absent in hippocampal organotypic cultures treated with the amyloid peptide $A_{25-35}$. Brain Res., 907, 188-194 (2001) https://doi.org/10.1016/S0006-8993(01)02517-3
  2. Bonde C., Noraberg J., and Zimmer J., Nuclear shrinkage and other markers of neuronal cell death after oxygen-glucose deprivation in rat hippocampals slice cultures. Neurosci. Lett., 327, 49-52 (2002) https://doi.org/10.1016/S0304-3940(02)00382-8
  3. Frotscher, M., Zafirow, S., and Heimrich, B., Development of identified neuronal types and of specific synaptic connections in slice cultures of rat hippocampus. Prog. Neurobiol., 45, 728 (1995)
  4. Fujikawa, T., Soya, H., Hibasami, H., Kawashima, H., Takeda, H., Nishibe, S., and Nakashima, K., Effect of Acanthopanax senticosus Harms on biogenic monoamine levels in the rat brain. Phytother. Res., 16, 474-478 (2002) https://doi.org/10.1002/ptr.1024
  5. Gahwiler, B.H., Capogna, M., Debanne, D., McKinney, R.A, and Thompson, S.M., Organotypic slice cultures: a technique has come of age. Trends Neurosci., 20, 471-477 (1997) https://doi.org/10.1016/S0166-2236(97)01122-3
  6. Gunasekar, P.G., Kanthasamy, A.G., Borowitz, J.L., and Isom, G. E., NMDA receptor activation produces concurrent generation of nitric oxide and reactive oxygen species: implication for cell death. J. Neurochem., 65, 2016-2021 (1995) https://doi.org/10.1046/j.1471-4159.1995.65052016.x
  7. Jung, H.J., Park, H.J., Kim, R.G., Shin, K.M., Ha, J., Choi, J.W.,, Kim, H.J., Lee, Y.S., and Lee, K.T., In vivo anti-inflammatory and antinociceptive effects of liriodendrin isolated from the stem bark of Acanthopanax senticosus. Planta Med., 69, 610-616 (2003) https://doi.org/10.1055/s-2003-41127
  8. Kristensen, B.W., Noraberg, J, and Zimmer, J, Comparison of excitotoxic profiles of ATPA, AMPA, KA and NMDA in organotypic hippocampal slice cultures. Brain Res., 917, 21-44 (2001) https://doi.org/10.1016/S0006-8993(01)02900-6
  9. Lee J, Son, D., Lee, P., Kim, S.Y, Kim, H., Kim, C.J., and Lim, E., Alkaloid fraction of Uncaria rhynchophylla protects against N-methyl-D-aspartate-induced apoptosis in rat hippocampal slices. Neurosci. Lett., 348, 51-55 (2003b) https://doi.org/10.1016/S0304-3940(03)00613-X
  10. Lee, J., Son, D., Lee, P., Kim, D.K, Shin, M.C., Jang, M.H., Kim, C.J., Kim, Y.S., Kim, S.Y., and Kim, H., Protective effect of methanol extract of Uncaria rhynchophylla against excitotoxicity induced by N-methyl-D-aspartate in rat hippocampus. J. Pharmacol. Sci., 92, 70-73 (2003c) https://doi.org/10.1254/jphs.92.70
  11. Lee, S., Shin, D.S., Oh, K.B., and Shin, K.H., Antibacterial compounds from the leaves of Acanthopanax senticosus. Arch. Pharm. Res., 26, 40-42 (2003a) https://doi.org/10.1007/BF03179929
  12. Lee, S., Son, D., Ryu, J., Lee, Y.S., Jung, S.H., Kang, J, Lee, S. Y, Kim, H.S., and Shin, K.H., Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch. Pharm. Res., 27, 106-110 (2004) https://doi.org/10.1007/BF02980055
  13. Li, X.C, Barnes, D., and Khan, I.A., A new lignan glycoside from Eleutherococcus senticasus. Planta Med., 67, 776-778 (2001) https://doi.org/10.1055/s-2001-18352
  14. Nicholas J., Willmott, Kay Wong and Anthony J. Strong., Intercellular $Ca^{2+}$ waves in rat hippocampal slice and dissociated glial-neuron cultures mediated by nitric oxide., FEBS Lett., 487, 239-247 (2000) https://doi.org/10.1016/S0014-5793(00)02359-0
  15. Nicholls, D. and Attwell, D., The release and uptake of excitatory amino acid. Trends Pharmacol. Sci., 11, 462-468 (1990) https://doi.org/10.1016/0165-6147(90)90129-V
  16. Noraberg, J., Kristensen, B.W., and Zimmer, J., Markers for neuronal degeneration in organotypic slice cultures. Brain Res. Protoc., 3, 278-290 (1999) https://doi.org/10.1016/S1385-299X(98)00050-6
  17. Pizzi, M., Consolandi, O., Memo, M., and Spano, P., N-Methyl-Daspartate neurotoxicity in hippocampal slice: protection by aniracetam. Eur. J. Pharmacol., 275, 311-314 (1995) https://doi.org/10.1016/0014-2999(95)00047-O
  18. Ryu, J., Son D., Kang, J., Kim, H.-S., Kim, B.-K., Lee, S., A benzenoid from the stem of Acanthopanx senticasus. Arch. Pharm. Res., 27, 912-914 (2004) https://doi.org/10.1007/BF02975842
  19. Tong, C.M., Shepherd, D., and Jahr, C.E., Synaptic desensitization of NMDA receptors by calcineurin. Science, 267, 1510-1512 (1995) https://doi.org/10.1126/science.7878472