The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effects of Orostachys japonicus on HepG2 Cell Proliferation and Oncogene Expression

와송(瓦松)이 HepG2 cell의 세포분열 및 관련유전자 발현에 미치는 영향

  • Moon, Young-Hun (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Young-Chul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Jang-Hoon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Woo, Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 문영훈 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2005.03.30
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Abstract

Objectives : The aim of the study was to evaluate the effect of WS on HepG2 cell cycle and expression of related genes. Methods : The MTT assay, Cell counting analysis, $[^3H]-Thymidine$ Incorporation Assay, Flow cytometric analysis, Quantitative RT-PCR were studied. Results : WS inhibited HepG2 cell proliferation in low concentration$(1-10\;{\mu]g/ml)$ which did not cause direct cytotoxicity, with dose-dependant manner. WS in-hibited DNA synthesis as well. Flow cytometric analysis on the HepG2 cell showed G2/M phase arrest. Conclusion : These results suggest that WS inhibits HepG2 cell proliferation not by the gene regulation but by G2/M phase arrest in the cell cycle. Thus further studies on the effect of WS in G2/M phase regulation are thought to be needed.

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