Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Nematicidal Activity and Chemical Component of Poria cocos

  • Li, Guo-Hong (Laboratory for Conservation and Utilization of Bio-resource, Yunnan University) ;
  • Shen, Yue-Mao (State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences) ;
  • Zhang, Ke-Qin (Laboratory for Conservation and Utilization of Bio-resource, Yunnan University)
  • Published : 2005.02.28
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Abstract

Poria cocos, a famous traditional Chinese medicine, was found to have nematicidal activity in experiments searching for nematicidal fungi. The experiment showed it could kill 94.9% of the saprophytic nematode, Panagrellus redivivue, 92.6% of the root-knot nematode, Meloidogyne arenaria, and 93.5% of the pine nematode, Bursaphelenchus xylophilus, on PDA plate within 12 hours. According to the nematicidal activity, three new compounds, 2, 4, 6-triacetylenic octane diacid, 2, 4, 5, 6-tetrahydroxyhexanoic acid and 3, 4-dihydroxy-2-keto-n-butyl 2,4,5,6-tetrahydroxyhexanate, were isolated from submerged cultures of Poria cocos. Of these, 2, 4, 6-triacetylenic octane diacid could kill 83.9% Meloidogyne arenaria and 73.4% Panagrellus redivivus at 500 ppm within 12 hours. Here, it is reported for the first time that Poria cocos has nematicidal activity.

Keywords

References

  1. Allan, F.R., B.A. Butt, and T. Jermy. 1980. Ployacetles from the rabbitbrush Chrysothamnus nauseosus. Phytochemistry. 19, 563-566 https://doi.org/10.1016/0031-9422(80)87015-4
  2. Anke, H. and O. Sterner. 1997. Nematicidal metabolites from higher fungi. Current organic chemistry. 1, 361-374
  3. Barron, G.L. and R.G. Thorn. 1987. Destruction of nematodes by species of Pleurotus. Can. J. Bot. 65, 774-778 https://doi.org/10.1139/b87-103
  4. Chang Q.X. and L.X. Ding. 2002. Analytic Manual of Active Component in Traditional Chinese Medicine, p. 1619-1622. XueYuan- Press, China
  5. Gray, N.F. 1984. Ecology of nematophagous fungi: Comparison of the soil sprinkling method with the Baerman funnel technique in the isolation of endoparasites. Soil Biol. Biochem. 16, 81-83 https://doi.org/10.1016/0038-0717(84)90131-7
  6. Kerry, B.R. and J.M. Bourne. 2002. A manual for research on Verticillium chlamydosporium, a potential biological control agent for root-knot nematodes, p.171. Druckform GmbH Merckstr, Germany
  7. Kimura, Y., M. Mori, A. Suzuki, and A. Kobayashi. 1981. Isolation and identification of two nematicidal substances from roots of Erigeron Philadelphicus L. and nematicidal acitvities of their related compounds. Agric. Biol. Chem. 45, 2915-2917 https://doi.org/10.1271/bbb1961.45.2915
  8. Kwok, O.C.H., R. Plattner, D. Weisleder, and D.T. Wicklow. 1992. A nematicidal toxin from Pleurotus ostreatus NRRL 3526. Journal of Chemical Ecology. 18(2), 127-136 https://doi.org/10.1007/BF00993748
  9. Mori, M., S. Hyeon, Y. Kimura, and A. Suzuki. 1982. The nematicidal activity of acetylene compounds. Agric. Biol. Chem. 46, 309-311 https://doi.org/10.1271/bbb1961.46.309
  10. Thorn, R.G. and G.L. Barron. 1984. Carnivorous mushrooms. Science. 224, 76-78 https://doi.org/10.1126/science.224.4644.76
  11. Tunlid, A., J. Ahman, and R.P. Oliver. 1999. Transformation of the nematode-trapping fungus Arthrobotrys oligospora. FEMS Microbiology Letters. 173, 111-116 https://doi.org/10.1111/j.1574-6968.1999.tb13491.x