Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
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Microarray를 이용한 Octachlorostyrene-노출 송사리(Oryzias latipes)에서의 분자생물학적 지표연구

Molecular Biomarkers of Octachlorostyrene Exposure in Medaka, Oryzias latipes, using Microarray Technique

  • 유대은 (동덕여자대학교 약학대학) ;
  • 강미선 (동덕여자대학교 약학대학) ;
  • 박은정 (동덕여자대학교 약학대학) ;
  • 김일찬 (한국해양연구원 극지연구소) ;
  • 이재성 (한양대학교 대학원 생물학과) ;
  • 박광식 (동덕여자대학교 약학대학)
  • You Dae-Eun (College of Pharmacy, Dongduk Women's University) ;
  • Kang Misun (College of Pharmacy, Dongduk Women's University) ;
  • Park Eun-Jung (College of Pharmacy, Dongduk Women's University) ;
  • Kim IL-Chan (Korea Polar Research Institute) ;
  • Lee Jae-Seong (Department of Environmental Science, Hanyang University) ;
  • Park Kwangsik (College of Pharmacy, Dongduk Women's University)
  • 발행 : 2005.06.01
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초록

Octachlorostyrene (OCS) is a primarily concerning chemical in many countries because of its persistent and bioaccumulative properties in the environment. OCS is not commercially manufactured or used but it may be produced during incineration or chemical synthetic processes involving chlorinated compounds. There are several reports that OCS was found in the waters, sediments, fish, mussels, and also in human tissues. However, systematic studies on the OCS toxicities are scarce in literature. In this study, we tried to get the gene expression data using medaka DNA chip to identify biomarkers of OCS exposure. Medaka (Oryzias latipes.) was exposed to OCS 1 ppm for 2 days and 10 days, respectively. Total RNA was extracted and purified by guanidine thiocyanate method and the Cy3- and Cy5-labelled cDNAs produced by reverse trancription of the RNA were hybridized to medaka microarray. As results, eighty five genes were found to be down-or up regulated by OCS. Some of the genes were listed and confirmed by real-time PCR.

키워드

참고문헌

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