Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Identification of Actinobacillus actinomycetemcomitans Using Species-Specific 16S rDNA Primers

  • Kim Su Gwan (Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University) ;
  • Kim Soo Heung (Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University) ;
  • Kim Mi Kwang (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kim Hwa Sook (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kook Joong Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University)
  • Published : 2005.04.01
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Abstract

The purpose of this study was to develop species-specific PCR primers for use in the identification and detection of Actinobacillus actinomycetemcomitans. These primers target variable regions of the 168 ribosomal RNA coding gene (rDNA). We assessed the specificity of the primers against 9 A. actinomycetemcomitans strains and 11 strains (3 species) of the Haemophilus genus. Primer sensitivity was determined by testing serial dilutions of the purified genomic DNAs of A. actinomycetemcomitans ATCC$ 33384^$T Our obtained data revealed that we had obtained species-specific amplicons for all of the tested A. actinomycetemcomitans strains, and that none of these amplicons occurred in any of the other species. Our PCR protocol proved able to detect as little as 4 fg of A. actinomycetemcomitans chromosomal DNA. Our findings suggest that these PCR primers are incredibly sensitive, and should prove suitable for application in epidemiological studies, as well as the diagnosis and monitoring of periodontal pathogens after treatment for periodontitis.

Keywords

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