Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

  • Yoo So Young (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kim Pyung Sik (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Hwan Ho Keel (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Lim Seong Hoon (Department of Orthodontics, College of Dentistry, Chosun University) ;
  • Kim Kwang Won (Department of Orthodontics, College of Dentistry, Chosun University) ;
  • Choe Son Jin (Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University) ;
  • Min Byung Moo (Department of Oral Biochemistry, College of Dentistry, Seoul National University) ;
  • Kook Joong Ki (Department of Oral Biochemistry, College of Dentistry, Seoul National University)
  • Published : 2005.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this study was to both isolate and identify non-mutans streptococci organisms (non­MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci.

Keywords

References

  1. Emilson, C.G. 1983. Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva. Scand. J. Dent. Res. 91, 26-32
  2. Farmer III, J.J. 1999. Enterobacteriaceae: Introduction and identification, p. 442-458. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds.), Manual of clinical microbiology. American Society for Microbiology, Washington, D.C
  3. Gold, O.G., H.V. Jordan, and J. van Houte. 1973. A selective medium for the isolation of Streptococcus mutans. Arch. Oral Biol. 18, 1357-1364 https://doi.org/10.1016/0003-9969(73)90109-X
  4. Hirasawa, M. and K. Takada. 2002. Susceptibility of Streptococcus mutans and Streptococcus sobrinus to cell wall inhibitors and development of a novel selective medium for S. sobrinus. Caries Res. 36, 155-160 https://doi.org/10.1159/000059329
  5. Hirasawa, M. and K. Takada. 2003. A new selective medium for Streptococcus mutans and the distribution of S. mutans and S. sobrinus and their serotypes in dental plaque. Caries Res. 37, 212-217 https://doi.org/10.1159/000070447
  6. Jensen, B. and D. Bratthall. 1989. A new method for the estimation of mutans streptococci in human saliva. J. Dent. Res. 68, 468-471 https://doi.org/10.1177/00220345890680030601
  7. Kimmel, L. and N. Tinanoff. 1991. A modified mitis salivarius medium for a caries diagnostic test. Oral Microbiol. Immunol. 6, 275-279 https://doi.org/10.1111/j.1399-302X.1991.tb00491.x
  8. Krasse, B. 1966. Human streptococci and experimental caries in hamsters. Arch. Oral Biol. 11, 429-436 https://doi.org/10.1016/0003-9969(66)90107-5
  9. Lane, D.J. 1991. 16S/23S rRNA sequencing, p. 115-175. In E. Stackebrandt, and M. Goodfellow (eds.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Inc., New York
  10. Loesche, W.J. 1986. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50, 353-380
  11. Niven, Jr., C.F., K.L. Smiley, and J.M. Sherman. 1942. The hydrolysis of arginine by streptococci. J. Bacteriol. 43, 651-660
  12. Shklair, I.L. and H.J. Keene. 1974. A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Arch. Oral Biol. 19, 1079-1081 https://doi.org/10.1016/0003-9969(74)90099-5
  13. Shklair, IL. and H.J. Keene. 1976. Biochemical characterization and distribution of Streptococcus mutans in three diverse populations, p. 201-210. In H.M. Stiles, W.J. Loesche, and T.C. O'Brien (eds.), Microbial aspects of dental caries. Information Retrieval Inc., Washington, D.C
  14. Tanzer, J.M., A.C. Borjesson, L. Laskowski, A.B. Kurasz, and M. Testa. 1984. Glucose-sucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius-bacitracin agar for enumeration of Streptococcus mutans. J. Clin. Microbiol. 20, 653-659
  15. Van Palenstein Helderman, W.H., M. Ijsseldijk, and J.H. Huis in 't Veld. 1983. A selective medium for the two major subgroups of the bacterium Streptococcus mutans isolated from human dental plaque and saliva. Arch. Oral Biol. 28, 599-603 https://doi.org/10.1016/0003-9969(83)90007-9
  16. Whiley, R.A. and D. Beighton. 1998. Current classification of the oral streptococci. Oral Microbiol. Immunol. 13, 195-216 https://doi.org/10.1111/j.1399-302X.1998.tb00698.x