Functional Characterization of the Gene Encoding UDP-glucose: Tetrahydrobiopterin $\alpha$-Glucosyltransferase in Synechococcus sp. PCC 7942

  • Cha En Young (School of Biotechnology and Biomedical Science, Inje University) ;
  • Park Jeong Soon (School of Biotechnology and Biomedical Science, Inje University) ;
  • Jeon Sireong (School of Biotechnology and Biomedical Science, Inje University) ;
  • Kong Jin Seon (School of Biotechnology and Biomedical Science, Inje University) ;
  • Cho Yong Kee (School of Biotechnology and Biomedical Science, Inje University) ;
  • Ryu Jee Youn (Department of Biology, Chungnam National University) ;
  • Park Youn Il (Department of Biology, Chungnam National University) ;
  • Park Young Shik (School of Biotechnology and Biomedical Science, Inje University)
  • Published : 2005.04.01

Abstract

In this study, we attempted to characterize the Synechococcus sp. pee 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin a-glucosyltransferase (BGluT). 2D­PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O$_2$ exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells. These results suggested that, in Synechococcus sp. pee 7942, BH4-glucoside might be involved in photosynthetic photoprotection.

Keywords

References

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