Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Characterization of Marine Bacterium Producing Arylsulfatase

  • BYUN , DAE-SEOK (Division of Food Science and Biotechnology, Pukyong National University) ;
  • KIM, DOO-SANG (Division of Food Science and Biotechnology, Pukyong National University) ;
  • J. SAMUEL GODBER, (Department of Food Science, Louisiana State University and Agricultural Station) ;
  • NAM, SOO-WAN (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • OH, MYONG-JOO (Department of Fish Pathology, Yosu National University) ;
  • SHIM, HANG-SUN (Division of Food Science and Biotechnology, Pukyong National University) ;
  • KIM, HYEUNG-RAK (Division of Food Science and Biotechnology, Pukyong National University)
  • Published : 2004.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A bacterial strain capable of hydrolyzing sulfate ester bonds in p-nitrophenyl sulfate and agar was isolated from the Southeast coast of Korea. The isolated strain (AS6330) is aerobic, Gram-negative, rod-shaped, and motile. Octadecanoic acid was the major cellular fatty acid in the isolate. An almost complete 16S rDNA sequence of the isolate was determined and the sequence similarity of the 16S rDNA with those of known Sphingomonas spp. was found to be at most $96.4\%$, implying that the isolate was a new Sphingomonas species. The organism was grown optimally at NaCl concentration of $1.5-3.5\%$. Optimum culture conditions were determined to be $30^{\circ}C$ and pH 7.0 for 48 h fermentation using a laboratory fermentor under constant culture conditions. Partially purified arylsulfatase through Q-Sepharose and phenyl­Sepharose chromatographies catalyzed hydrolysis of sulfate ester bonds in agar, and $97\%$ of sulfates in agar were removed after 4 h reaction at $45^{\circ}C$ and pH 7.0. The arylsulfatase from the isolated bacterium might be useful for the removal of sulfate groups in agar.

Keywords

References

  1. Barbeyron, T., P. Potin, C. Richard, O. Collin, and B. Kloareg. 1995. Arylsulfatase from Alteromonas carrageenovora. Microbiology 141: 2897-2904
  2. Beil, S., H. Kehrli, J. Peter, W. Staudenmann, A. M. Cook, T. Leisinger, and M. A. Kertesz. 1995. Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA). Eur. J. Biochem. 229: 385-394
  3. Chung, W. K. and G. M. King. 2001. Isolation, characterization, and polyaromatic hydrocarbon degradation potential of aerobic bacteria from marine macrofaunal burrow sediments and description of Lutibacterium anuloederans gen. nov., sp. nov., and Cycloclasticus spirillensus sp. nov. Appl. Environ. Microbiol. 67: 5585-5592
  4. Dodgson, K. S. and R. G. Price. 1962. A note on the determination of the ester sulfate content of sulfated polysaccharides. Biochem. J. 84: 350-356
  5. Edward, U., T. Rogall, H. Blocker, M. Emde, and E. C. Bottger. 1989. Isolation and direct complete determination of entire genes. Nucleic Acids Res. 17: 7843-7853
  6. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791
  7. Felsenstein, J. 1993. PHYLIP (phylogenetic inference package) version 3.5c. Seattle, University of Washington, U.S.A
  8. Fitch, W. M. and E. Margoliash. 1967. Construction of phylogenetic trees. Science 155: 279-284
  9. Gonzalez, J. M. and M. A. Moran. 1997. Numerical dominance of a group of marine bacteria in the $\alpha$-subclass of the class Proteobacteria in coastal seawater. Appl. Environ. Microbiol. 63: 4237-4242
  10. Gonzalez, J. M., F. Mayer, M. A. Moran, R. E. Hodson, and W. B. Whitman. 1997. Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. Int. J. Syst. Bacteriol. 47: 773-780
  11. Hoshi, M. and T. Moriya. 1980. Arylsulfatase of sea-urchin sperm. 2. Arylsulfatase as a lysine of sea-urchins. Dev. Biol. 74: 343-350
  12. Iwamoto, Y., R. Araki, K. Iriyama, T. Oda, H. Fukuda, S. Hayashida, and T. Muramatsu. 2001. Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. Biosci. Biotechnol. Biochem. 65: 133-142
  13. Jukes, T. S. and C. R. Cantor. 1969. Evolution of protein molecules, pp. 21-132. In H. N. Munro (ed.), Mammalian Protein Metabolism. Academic Press, New York, U.S.A
  14. Kertesz, M. A. 1999. Riding the sulfur cycle-metabolism of sulfonates and sulfate esters in Gram-negative bacteria. FEMS Microbiol. Rev. 24: 135-175
  15. Kovacs, N. 1956. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178: 703
  16. Ledyard, K. M., E. F. DeLong, and J. W. H. Dacey. 1993. Characterization of a DMSP-degrading bacterial isolate from the Sargasso Sea. Arch. Microbiol. 160: 312-318
  17. Lee, J. S., D. S. Joo, S. Y. Cho, J. H. Ha, and E. H. Lee. 2000. Purification and characterization of extracellular chitinase produced by marine bacterium, Bacillus sp. LJ-25. J. Microbiol. Biotechnol. 10: 307-311
  18. Marzluf, G. A. 1997. Molecular genetics of sulfur assimilation in filamentous fungi and yeast. Annu. Rev. Microbiol. 51: 73-96 https://doi.org/10.1146/annurev.micro.51.1.73
  19. Miech, C., T. Dierks, T. Selmer, K. von Figura, and B. Schmidt. 1998. Arylsulfatase from Klebsiella pneumoniae carries a formylglycine generated from a serine. J. Biol. Chem. 273: 4835-4837
  20. Milanesi, A. A. and J. W. C. Bind. 1972. Lysosomal enzymes in aquatic species II. Distribution and particle properties of thermally acclimated muscle lysosomes of rainbow trout Salmo gairdeneri. Comp. Biochem. Physiol. 41: 473-491
  21. Murooka, Y. and T. Harada. 1981. Regulation of derepressed synthesis of arylsulfatase by tyramine oxidase in Salmonella typhimurium. J. Biol. Chem. 145: 796-802
  22. Roberton, A. M. and D. P. Wright. 1997. Bacterial glycosulfatases and sulphomucin degradation. Can. J. Gastroenterol. 11: 361-366
  23. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425
  24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A
  25. Selby, H. H. and R. L. Whistler. 1993. Agar, pp. 87-103. In Whistler, R. L. and BeMiller, J. N. (eds.), Industrial Gums. Academic Press, New York, U.S.A
  26. Seo, H. J., J. H. Kim, D. S. Byun, and H. R. Kim. 2001. Purification and characterization of arylsulfatase produced from Sphingomonas sp. nov. pp. 1575-1578, 75th meeting of Japanese Society of Fisheries Science, Yokohama, Japan
  27. Sorokin, D. Y. 1995. Sulfitobacter pontiacus gen. nov., sp. nov.: A new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. Microbiology 64: 354-365
  28. Stackebrandt, E. and B. M. Goebel. 1994. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44: 846-849
  29. Vera, J., R. Alvarez, E. Murano, J. C. Slebe, and O. Leon. 1998. Identification of a marine agarolytic Pseudoalteromonas isolate and characterization of its extracellular agarase. Appl. Environ. Microbiol. 64: 4378-4383
  30. Wayne, L. G., D. J. Brenner, R. R. Colwell, P. A. D. Grimont, O. Kandler, M. I. Krichewsky, L. H. Moore, W. E. C. Moore, R. G. E. Murray, E. Stackebrandt, M. P. Starr, and H. G. Trueper. 1987. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463-464
  31. Woes, C. R. 1987. Bacterial evolution. Microbiol. Rev. 51: 221-271
  32. Yabuuchi, E., Y. Kosako, T. Naka, S. Suzuki, and I. Yano. 1999. Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown, 1990) Comb. nov., Sphingomonas natatoria (Sly, 1985) Comb. nov., Sphingomonas ursincola (Yurkov et al., 1997) Comb. nov., and emendation of the genus Sphingomonas. Microbial Immunol. 43: 339-349
  33. Yoon, H. S. and Y. H. Park. 1984. Studies on the composition of agarose and agaropectin in agar-agar. Bull. Korean Fish. Soc. 24: 27-33