Biotechnology and Bioprocess Engineering:BBE

  • 제9권6호
  • /
  • Pages.447-453
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  • 2004
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Effects of Modifiers on the Supercritical $CO_{2}$ Extraction of Glycyrrhizin from Licorice and the Morphology of Licorice Tissue after Extraction

  • Kim Hyun Seok (Department of Chemical and Biochemical Engineering, University of Suwon) ;
  • Lee Sang Yun (Department of Chemical and Biochemical Engineering, University of Suwon) ;
  • Kim Byung Yong (Department of Food Science and Technology, Kyunghee University) ;
  • Lee Eun Kyu (Department of Chemical Engineering, Hanyang University) ;
  • Ryu Jong Hoon (Department of Chemical and Biochemical Engineering, University of Suwon) ;
  • Lim Gio Bin (Department of Chemical and Biochemical Engineering, University of Suwon)
  • 발행 : 2004.11.01
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초록

Optimal conditions for the supercritical carbon dioxide $(scCO_{2})$ extraction of glycyr­rhizin from licorice (Glycyrrhiza glabra) were investigated, with an emphasis on the types and levels of modifiers. The morphology of the licorice tissue remaining after the $scCO_{2} $ extraction of glycyrrhizin was examined by scanning electron microscopy, coupled with measurements of ab­solute density. Conventional organic solvent extraction was also carried out for purpose of quantitative comparison. At 50 MPa and $60^{circ}C$ glycyrrhizin could not be extracted with pure $scCO_{2}$, while a considerable amount of glycyrrhizin was extracted when water was added to $scCO_{2}$ as a modifier. The highest recovery was found to be about $97\%$ when $70\%$ aqueous methanol was added to $scCO_{2}$ at a concentration of $15\%$. The optimal pressure and temperature for the supercritical fluid extraction of glycyrrhizin were observed to be 30 M Pa and $60^{circ}C$, respectively. Under these conditions, the percentage recovery of glycyrrhizin attained a maximum value of 102.67\pm$ $1.13\%$ within 60 min. Furthermore, in the case of $scCO_{2}$ modified with $70\%$ aqueous methanol, the licorice tissue obtained after extraction was found to be severely de­graded by excessive swelling, and the absolute density of the licorice residues was observed to be the highest.

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참고문헌

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