Biotechnology and Bioprocess Engineering:BBE

  • 제9권6호
  • /
  • Pages.440-446
  • /
  • 2004
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Bipolar Heterothallism, a Principal Mating System of Cordyceps militaris In Vitro

  • Shrestha Bhushan (Department of Applied Biology, Kangwon National University) ;
  • Kim Ho Kyung (Mushtech Co. Ltd) ;
  • Sung Gi Ho (Department of Botany and Plant Pathology, Oregon State University) ;
  • Spatafora Joseph W. (Department of Botany and Plant Pathology, Oregon State University) ;
  • Sung Jae Mo (Department of Applied Biology, Kangwon National University)
  • 발행 : 2004.11.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Interest in in vitro study of entomopathogenic fungi, including Cordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. Among Cordyceps species, in vitro stromata of C militaris has been successfully produced and cultivated for industrial purposes. However, genetic study on in vitro stromata formation of C militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation of C militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, mono­ascospore cultures without combination produced either no stromata or only abnormal non­perithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains of C militaris. These observations indicated that C militaris behaves as a bipolar het­erothallic fungus and requires two mating compatible strains in order to produce regular club­shaped perithecial stromata, a fundamental requirement for its industrial cultivation.

키워드

참고문헌

  1. Kobayasi, Y. (1982) Keys to the taxa of the genera Cordyceps and Torrubiella. Trans. Mycol. Soc. Japan. 23: 329-364
  2. Hywel-Jones, N. L. (2002) Multiples of eight in Cordyceps ascospores. Mycol. Res. 106: 2-3 https://doi.org/10.1017/S0953756202235534
  3. Higashiyama, K., S. Fujikawa, E. Y. Park, and S. Shimizu (2002) Production of arachidonic acid by Mortierella fungi. Biotechnol. Bioprocess Eng. 7: 252-262 https://doi.org/10.1007/BF02932833
  4. Lee, B. K., H. Y. Piao, and W. J. Chung (2002) Production of red pigments by Monascus purpureus in solid-state culture. Biotechnol. Bioprocess Eng. 7: 21-25 https://doi.org/10.1007/BF02935875
  5. Lee, K. J. and J. Y. Lim (2003) Optimized conditions for high erythritol production by Penicillium sp. KJ-UV29, mutant of Penicillium sp. KJ81. Biotechnol.Bioprocess Eng. 8: 173-178 https://doi.org/10.1007/BF02935892
  6. Ryu, W. Y., M. Y. Jang, and M. H. Cho (2003) The selective visualization of lignin peroxidase, manganese peroxidase and laccase, produced by white rot fungi on solid media. Biotechnol. Bioprocess Eng. 8: 130-134 https://doi.org/10.1007/BF02940268
  7. de Bary, A. (1867) Zur kenntniss insectentodtender Pilze. Bot. Zeit. 25: 1-7, 9-13, 17-21
  8. de Bary, A. (1887) Comparative Morphology and Biology of the Fungi, Mycetozoa and Bacteria. pp. 359-374. Clarendon Press, Oxford, UK
  9. Sato, H. and M. Shimazu (2002) Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Appl. Entomol. Zool. 37: 85-92 https://doi.org/10.1303/aez.2002.85
  10. Pettit, R. H. (1895) Studies in artificial cultures of entomogenous fungi. Cornell Univ. Agri. Expt. Stn. Bot. Entomol. Div. Bull. 97: 339-378
  11. Sopp, J. O. O. (1911) Untersuchungen uber insektenvertilgende Pilze bei den letzten Kieferspinnerepidemien in Norwegen. Vidensk. Selsk. Kria. Skr. I. Math.-naturv. Kl. 2: 1-56
  12. Basith, M. and M. F. Madelin (1968) Studies on the production of perithecial stromata by Cordyceps militaris in artificial culture. Can. J. Bot. 46: 473-480 https://doi.org/10.1139/b68-071
  13. Kobayasi, Y. (1941) The genus Cordyceps and its allies. Sci. Rept. Tokyo. Bunrika. Daigaku. Sect. B. 5: 53-260
  14. Liang, Z. (1990) Anamorph of Cordyceps militaris and artificial culture of its fruibody. Southwest China J. Agri. Sci. 3: 1-6
  15. Pen, X. (1995) The cultivation of C. militaris fruitbody on artificial media and the determination of SOD. Acta Edulis. Fungi 2: 25-28
  16. Sung, J. M. (1996) Insect-Born Fungi of Korea. Kyo-Hak Publishing Co. Ltd., Seoul, Korea
  17. Choi, I. Y., J. S. Choi, W. H. Lee, Y. J. Yu, G. T. Joung, I. O. Ju, and Y. K. Choi (1999) The condition of production of artificial fruiting body of Crodyceps militaris. Kor. J. Mycol. 27: 243-248
  18. Sung, J. M, Y. S. Choi, H. K. Lee, S. H. Kim, Y. O. Kim, and G. H. Sung (1999) Production of fruiting body using cultures of entomopathogenic fungal species. Kor. J. Mycol. 27: 15-19
  19. Sung, J. M., Y. S. Choi, B. Shrestha, and Y. J. Park (2002) Investigation on artificial fruiting of Cordyceps militaris. Kor. J. Mycol. 30: 6-10 https://doi.org/10.4489/KJM.2002.30.1.006
  20. Sato, H. and M. Shimazu (2002) Homothallism in Cordyceps militaris. pp. 311. Book of Abstracts of the 7th International Mycological Congress. August 11-17. Oslo, Norway
  21. Sung, J. M. and B. Shrestha (2002) In vitro fruiting of Cordyceps militaris. pp. 113. Book of Abstracts of the 7th International Mycological Congress. August 11-17. Oslo, Norway
  22. Harris, S. D. (2001) Genetic analysis of ascomycete fungi. pp. 47-58. In: N. Talbot (ed.). Molecular and Cellular Biology of Filamentous Fungi: A Practical Approach. Oxford University Press, Oxford, UK
  23. Yokoyama, E., K. Yamagishi, and A. Hara (2003) Structures of the mating-type loci of Cordyceps takaomontana. Appl. Environ. Microbiol. 69: 5019-5022 https://doi.org/10.1128/AEM.69.8.5019-5022.2003
  24. McGuire, I. C, R. E. Marra, and M. G. Milgroom (2004) Mating-type heterokaryosis and selfing in Cryphonectria parasitica. Fung. Gen. Biol. 41: 521-533 https://doi.org/10.1016/j.fgb.2003.12.007
  25. Turgeon, B. G. and O. C. Yoder (2000) Proposed nomenclature for mating type genes of filamentous ascomycetes. Fung. Gen. Biol. 31: 1-5 https://doi.org/10.1006/fgbi.2000.1227
  26. Jung, G. T., I. O. Ju, Y. Z. Yu, J. Ryu, J. S. Choi, and Y. G. Choi (2003) Mycelial yield of Pleurotus ostreatus using thinned apple, pear, and peach on submerged culture. Biotechnol. Bioprocess Eng. 8: 286-290 https://doi.org/10.1007/BF02949218