Journal of Preventive Medicine and Public Health
- 제37권4호
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- Pages.321-328
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- 2004
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- 1975-8375(pISSN)
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- 2233-4521(eISSN)
크실렌 노출로 인한 요중 메틸마뇨산 배설에 미치는 유전자 다형성 연구
A Study on Polymorphism Affecting Excretion of Urinary Methylhippuric Acid due to Xylene Exposure
- 김청식 (서울대학교 의과대학 예방의학교실) ;
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고상백
(연세대학교 원주의과대학 예방의학교실 및 직업의학연구소) ;
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김형수
(건국대학교 의과대학 예방의학교실) ;
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박수경
(건국대학교 의과대학 예방의학교실) ;
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장성훈
(건국대학교 의과대학 예방의학교실)
- Kim, Cheong-Sik (Department of Preventive Medicine, Seoul National University College of Medicine) ;
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Koh, Sang-Baek
(Department of Preventive Medicine and Institute of Occupational Medicine, Yonsei University Wonju College of Medicine) ;
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Kim, Hyeong-Su
(Department of Preventive Medicine, College of Medicine, Konkuk University) ;
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Park, Sue-Kyung
(Department of Preventive Medicine, College of Medicine, Konkuk University) ;
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Chang, Soung-Hoon
(Department of Preventive Medicine, College of Medicine, Konkuk University)
- 발행 : 2004.12.01
초록
Objectives : The purpose of this study was to investigate the effect of genetic polymorphism of cytochrome P450 2E1 (CYP2E1) and aldehyde dehydrogenase 2 (ALDH2) on the xylene metabolism. Methods : Among 247 workers, 116 were occupationally exposed to xylene and 131 were not. Workers exposed to xylene had different work such as spray, touch-up, mix & assist, and pre-treat. Questionnaire variables were age, sex, use of personal protective equipment, smoking, previous night's drinking and work duration. The urinary methylhippuric acid was measured in the urine collected in the afternoon and corrected by urinary creatinine concentration. The genotypes of CYP2E1 and ALDH2 were investigated by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) methods with DNA extracted from venous blood. Results : 1. The urinary concentrations of o-, m-, and p-methylhippuric acid and total methylhippuric acid in the exposed group were significantly higher than those in the non-exposed group (p<0.001). 2. In multiple regression analysis, the urinary methylhippuric acid concentration was significantly influenced by exposure grade (Job-exposure matrixes), smoking, drug use and kind of protective equipment (p<0.1). 3. Genetic polymorphism of CYP2E1 and ALDH2 did not affect urinary methylhippuric acid level in the exposed group (p>0.05). Conclusions : Exposure grade, smoking, drug use and kind of protective equipment affected urinary methylhippuric acid level, whereas genetic polymorphism of CYP2E1 and ALDH2 did not. However, further investigation for the effect of genetic polymorphism on the metabolism of xylene with a larger sample size is needed.