Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of Enamel Matrix Drivatives application on the expression of PDLs17, PDLs22 of cultured human periodontal ligament cells in vitro

Enamel Matrix Derivatives가 사람 치주인대 세포의 특이유전자인 PDLs17, PDLs22의 발현에 끼치는 효과

  • Han, Geun-A (Dept, of periodontology, college of dentistry, Chosun University) ;
  • Jang, Hyun-seon (Dept, of periodontology, college of dentistry, Chosun University) ;
  • Kok, Jung-Ki (Dept, of biochemistry, college of dentistry, Chosun University) ;
  • Park, Ju-Chol (Dept, of oral histology, college of dentistry, Chosun University) ;
  • Kim, Heoung-Jung (Dept, of anatomy, college of dentistry, Chosun University) ;
  • Kim, Jung-Gwan (Dept, of periodontology, college of dentistry, Yunsei University) ;
  • Kim, Byung-Ock (Dept, of periodontology, college of dentistry, Chosun University)
  • 한근아 (조선대학교 치과대학 치주과학 교실) ;
  • 장현선 (조선대학교 치과대학 치주과학 교실) ;
  • 국중기 (조선대학교 치과대학 생화학 교실) ;
  • 박주철 (조선대학교 치과대학 구강조직 발생학 교실) ;
  • 김흥중 (조선대학교 치과대학 해부학 교실) ;
  • 김종관 (연세대학교 치과대학 치주과학 교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학 교실)
  • Published : 2004.06.30
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Abstract

The enamel matrix derivative (EMD) has been recently used in the periodontal regenerative techniques. The present study was established to investigate the influence of EMD on human periodontal ligament cells using expression of mRNA of periodontal ligament specific gene (PDLs)17, PDLs22, type I collagen when EMD applied to periodontal ligament cells. Periodontal ligament cells were obtained from a healthy periodontium and cultured in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum and ${\beta}-glycerophosphate$ with ascorbic acid. Test groups were two; One adds EMD in culture media and another added EMD and Dexamethasone (DEX) in culture media. Positive control group added DEX in culture media, and negative control group adds niether of EMD nor DEX. $Emdogain^{(R)}$ (Biora, Sweden, 30 mg/ml) was diluted by 75 ${\mu}g/ml$ concentration to culture media. For reverse transcription-polymerase chain reaction (RT-PCR), total RNA isolated on days 0, 7, 14 and 21. mRNA of PDLs17 was expressed on days 14 and 21 in EMD or DEX group, and expressed on days 7, 14 and 21 in EMD plus DEX group, the other side, expressed on days 21 in negative control group. mRNA of PDLs22 expressed on days 7, 14 and 21 in EMD group, and expressed on days 14 and 21 in DEX group, and expressed on days 7, 14 and 21 in EMD plus DEX group. Negative control group expressed on days 14 and 21. Type I collagen was expressed on all days and all groups. These results indicate that EMD promotes differentiation of periodontal ligament cells, and this is considered to offer basis that can apply EMD to periodontal tissue regeneration technique.

Keywords

References

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