Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Study on the Biological Characteristics of Cultured Osteoblasts Derived from Alveolar Bone

배양 치조골모세포의 생물학적 특성에 관한 연구

  • Lee, Yong-Bae (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lee, Seong-Jin (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Suk-Joo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Seong-Yun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Sin, Gye-Cheol (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Hyun-A (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 이용배 (원광대학교 치과대학 치주과학교실) ;
  • 이성진 (원광대학교 치과대학 치주과학교실) ;
  • 유석주 (원광대학교 치과대학 치주과학교실) ;
  • 김성윤 (원광대학교 치과대학 치주과학교실) ;
  • 신계철 (원광대학교 치과대학 치주과학교실) ;
  • 김현아 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실)
  • Published : 2004.06.30
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Abstract

Osteoblasts from alveolar bone may have an important role in the bone regeneration for periodontium, but their culture and characterization are not determined yet. The purpose of this study was to investigate the biological characteristics of primary explant cultured osteoblasts(PECO) from alveolar bone. Osteoblasts were isolated and cultured from alveolar socket of extracted tooth in children. To compare the characteristics, osteoblasts and gingival fibroblasts were cultured with DMEM at $37^{\circ}C$, 5% $CO_2$, l00% humidity incubator, and human fetal osteoblasts cell line(hFOB1) were cultured with DMEM at $34^{\circ}C$, 5%, $CO_2$ 100% humidity incubator. To characterize the isolated bone cells, morphologic change, cell proliferation and differentiation were measured. Morphology of PECO was small round body or cuboidal shape on inverted microscope and was similar with hFOB1. PECO became polygonal shape with stellate and had an amorphous shape at 9th passage in culture. PECO had significantly higher activity than that of gingival fibroblasts and hFOB1 in alkaline phosphatase activity. The expression of osteocalcin and bone sialoprotein in PECO was notably increased when compared with hFOB1 and gingival fibroblasts. These result indicated that PECO from alveolar bone in children has an obvious characteristics of osteoblast, maybe applied for the regeneration of bone.

Keywords

References

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