Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Ethionine Resistance Conferring Gene in an Industrial Strain of Saccharomyces cerevisiae

산업용 Saccharomyces cerevisiae에서 Ethionine 저항성 유전자의 발현

  • Park, Jeong-Nam (Department of Biological Sciences, Chonnam National University) ;
  • Lee, Gyeong-Hui (Department of Biological Sciences, Chonnam National University) ;
  • Go, Hyeon-Mi (Department of Biological Sciences, Chonnam National University) ;
  • Seo, Guk-Heon (Department of Biological Sciences, Chonnam National University) ;
  • Jin, Jong-Eon (Department of Cosmetology, Dongkang College) ;
  • Lee, Hwang-Hui (Department of Biological Sciences, Chonnam National University) ;
  • Bae, Seok (Department of Biological Sciences, Chonnam National University)
  • 박정남 (전남대학교 생명과학부) ;
  • 이경희 (전남대학교 생명과학부) ;
  • 고현미 (전남대학교 생명과학부) ;
  • 서국헌 (전남대학교 생명과학부) ;
  • 진종언 (동강대학 피부미용과) ;
  • 이황희 (전남대학교 생명과학부) ;
  • 배석 (전남대학교 생명과학부)
  • Published : 2004.12.01
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Abstract

The ethionine resisconferring gene (ERCI) was constitutively expressed under the control of the alcohol dehydrogenase gene promoter (ADClp) and introduced into the chromosomes of an industrial polyploid strain of Saccharocerevisiae by using the 8-sequences of the Tyl retrotransposon as the recombination site. 8-Integrative cassette devoid of bacterial DNA sequences containing the ampicillin resistance gene was constructed that had the aureobasidin A resistance gene (AURl-C) as the selection marker and ERCl gene. The ERCl gene was also employed as the selection marker in the 8-integrative cassette lacking the A URl-C gene. Industrial Saccerevisiae transformed with these integrative cassettes exhibited strong resistance to DL-ethioncompared with nontransformants.

Keywords

References

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