Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hydrolysis of Galactomannan and Manno-oligosaccharides by A Bacillus subtiis Mannanase

Bacillus subtilis의 mannanase에 의한 갈락토만난과 만노올리고당의 가수분해

  • Gwon, Min-A (School of Applied Food and Nutritional Science, Woosong University) ;
  • Yun, Gi-Hong (Bioresouce and Application Research Center, Woosong University)
  • 권민아 (우송대학교 응용식품 영양학부) ;
  • 윤기홍 (생물소재응용연구센터)
  • Published : 2004.12.01
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Abstract

Hydrolysis of manno-oligosaccharides and galactomannan was studied with the purified Bacillus subtilis WL-7 mannanase from recombinant Eschericoli. The predominant products of hydrolysis were mannose, mannobiose and mannotriose. The enzyme could hydrolyze $\beta$-1 A-linked manno-oligosaccharides larger than mannobiose, but was not active on mannobiose. When the mannanase hydrolyzed manno-oligo saccharides of degree of polymerization(DP) 4-6, it was more active on the substrate of higher DP. Based on analysis of transient reaction products by TLC, the enzyme was found to have a preference for internal $\beta$-IA-mannosidic linkages, which are the central mannosidic bond of mannotetraose and the two middle mannosidic bonds of mannopentaose. The $\beta$-l A-mannosidic bonds situated at the second and fourth positions from the nonreducing end of mannohexaose were preferenhydrolyzed by the mannanase. Locust bean gum(LBG) was enzymatically hydrolyzed with higher efficiency than guar gum, resulting that amount of reducing sugars was liberated more efficiently from LBG than guar gum with same activity of mannanase.

Keywords

References

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