International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Cloning of Small Plasmids from Bacillus thuringiensis Subsp. israelensis Using Plasmid Capture System

  • Choi, Jae Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Roh, Jong Yul (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Li, Ming Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Shim, Hee Jin (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kang, Joong Nam (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Woo, Soo Dong (Department of Agricultural Biology, Chungbuk National University) ;
  • Jin, Byung Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Je, Yeon Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
  • Published : 2004.12.01
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Abstract

Recently, we have developed an easy, simple and convenient circular DNA cloning system named plasmid capture system (PCS). To investigate usefulness of PCS in cloning of plasmids from Bacillus thuringiensis strains, PCS donors, pPCS-S and pPCS-L were applied to clone plasmids of B. thuringiensis subsp. israelensis by in vitro transposition using 4{TnsABC^*}$ transposase. In result, 3 small plasmids were cloned, and these were consistent with pTX14-1, pTX14-2 and pTX14-3 reported previously from B. thuringiensis subsp. israelensis. Therefore, the PCS can be successfully applied to clone small plasmids from B. thuringiensis strains.

Keywords

References

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