분석과학 (Analytical Science and Technology)

  • 제17권2호
  • /
  • Pages.192-197
  • /
  • 2004
  • /
  • 1225-0163(pISSN)
  • /
  • 2288-8985(eISSN)
한국분석과학회 (The Korean Society of Analytical Sciences)
권호 표지

Capillary electrophoresis를 이용한 한국산 및 중국산 고사리의 원산지 판별방법 개발

Development of the Discrimination Methods for Geographical Origin of Bracken(Pteridium aquilinum) by Capillary Electrophoresis

  • 투고 : 2003.10.16
  • 심사 : 2004.01.16
  • 발행 : 2004.04.25
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초록

The discrimination of bracken (Korean vs. Chinese) has been attempted using capillary electrophoresis (CE). Bracken (Pteridium aquilinum) was extracted with 30% methanol and separated on a uncoated fused-silica ($50{\mu}m{\times}27cm$) capillary. Conditions for optimal analysis include: temperature, $40^{\circ}C$; voltage, 8 kV; and pressure injection time, 5 sec. The optimal separation buffer was 0.3 M borate buffer (pH 8.5) containing 40 mM CHAPS with 30% ethylene glycol. Under the optimal conditions established for CE, the ratio of specific peak area (peak PA-1) to other peak area (peak PA-2) was effective in discrimination of Korean and Chinese bracken. The mean accuracy for discrimination of Korean and Chinese brackens were 80% and 86%, respectively.

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