Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Expression of Ids in Preimplantation Mouse Embryos

착상 전 생쥐배아에서 Id 유전자의 발현

  • Hong, Seok-Ho (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Nah, Hee-Young (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Lee, Young-Jin (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Lee, Ji-Won (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Son, Young-Soo (Cheong-Ju St. Marys' Hospital) ;
  • Chae, Hee-Dong (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Kim, Sung-Hoon (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Kang, Byung-Moon (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center) ;
  • Kim, Chung-Hoon (Dept of OBGY, College of Medicine, Ulsan University, Asan Medical Center)
  • 홍석호 (울산대학교 서울아산병원 산부인과) ;
  • 나희영 (울산대학교 서울아산병원 산부인과) ;
  • 이영진 (울산대학교 서울아산병원 산부인과) ;
  • 이지원 (울산대학교 서울아산병원 산부인과) ;
  • 손영수 (청주성모병원) ;
  • 채희동 (울산대학교 서울아산병원 산부인과) ;
  • 김성훈 (울산대학교 서울아산병원 산부인과) ;
  • 강병문 (울산대학교 서울아산병원 산부인과) ;
  • 김정훈 (울산대학교 서울아산병원 산부인과)
  • Published : 2004.12.30
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Abstract

Objective: The Id family of helix-loop-helix proteins are thought to affect the balance between cell growth and differentiation by negatively regulating the function of basic-helix-loop-helix (bHLH) transcriptional factors. The aim of this study was to investigate the expression pattern of Ids (Id-1, -2, -3, and -4) in preimplantation mouse embryos at mRNA and protein levels. Methods: Oocytes and preimplantation embryos were collected from reproductive organs of female ICR mice following superovulation. RT-PCR was performed to investigate the mRNA expression patterns of Id genes and their protein were localized by immunofluorescence analysis. Results: Id-1 and Id-3 mRNAs were strongly expressed at the germinal vesicle (GV) oocyte and the blastocyst stages. Id-2 mRNA was expressed throughout preimplantation embryo development, but Id-4 was not expressed. Immunofluorescence showed that Id-1 and Id-2 were predominantly localized in cytoplasmic region, but the immunofluorescence signal of Id-3 was weak throughout preimplantation embryo development. Conclusion: These data show for the first time that Ids are expressed in preimplantation mouse embryos and suggest that Ids may play an important role in early preimplantation embryo development and uterine physiological changes.

Keywords

References

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