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The antifungal activity and growth promotion effects of Bacillus sp. LP03, TBM40-3 on Pohang Buchu (Leeks).

포항 부추에 대한 biosurfactant를 생산하는 Bacillus sp. LP03, TBM40-3의 항진균성과 생육에 미치는 영향

  • 장혜원 (한동대학교 생물 식품 과학부) ;
  • 최용락 (동아대학교 생명자원과학부) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 최윤혁 (한동대학교 생명공학 연구소) ;
  • 도형기 (한동대학교 생명공학 연구소) ;
  • 황철원 (한동대학교 생명공학 연구소)
  • Published : 2004.10.01

Abstract

This report investigates antifungal activity and effects of growth promotion by biosurfactant produced from Bacillus sp. LP03 and TBM40-3 against fungus causing plants disease (Glay Mold-Botrytis cinerea). Antifugal activity against B. cinerea infeeted to leek (Allium tuberosum Rottler) exhibited better than antifungal agent farming drug (smilex, Dong bang agro., Seoul, Korea.) through the field test. After infected by plant's disease, the leaves growth and number are maintained under presenting biosurfactant produced strains. Especially, one of the strains, named Bacillus sp. LP03 showed strong antifungal activity on field studies.

본 연구에서는 두 균주, Bacillus sp. LP03, TBM40-3의 biosurfactan에 대한 생육 시 적절한 온도와 유화활성에 대한 실험을 행한 후 가장 적절한 조건에서 배양하였고, 이미 항진균성과 유화활성에 대해 실험실 수준에서 검증된 TBM40-3뿐 아니라 분리되어 보관되어 있는 균주, LP03 또한 실험실 수준에서 항진균성 및 유화활성이 확인되었고 이후에 시행한 부추에 생기는 병인 B. cinerea에 대한 항진균성 및 생육조사를 시행한 결과 이미 항진균성과 유화활성이 확인되어있던 TBM40-3에 비해 그 효과가 우수할 뿐 아니라 생육 촉진효과를 확인할 수 있었다.

Keywords

References

  1. Borjana, K. Tuleva, George R. Ivanov and Nelly E. Christova., 2002. Biosurfactant Production by New Pseudomonas putida Strain., Z. Naturforsch, 57c, 356-360
  2. Cairns, W. L., D. G. Cooper, J. E. Zajic, J. M, Wood, and N. Kosaric. 1892. Characterization of Nocardia amaree as a potent biological coalescing agent of water-oil emlsions. Appl. Environ. Microbiol., 43, 362-366
  3. Deleu M., and M. Paquot, 2004. From renewable vegetable resources to microorganism : new trends in surfactants, C. R. Chimie, 7, 641-646 https://doi.org/10.1016/j.crci.2004.04.002
  4. Folman, L. B., M.J.E.M. De Klein, J. Postma, and J. A. van Veen, 2004. Production of antifungal compounds by Lysobacter enzymogenes isolate 3.1T8 under different conditions in relation to its efficacy as a biocontrol agent of Pythium aphanidermatum in cucumber, Biological Control, 31, 145-154 https://doi.org/10.1016/j.biocontrol.2004.03.008
  5. Georgiou, G., S. C. Lin, and M. M. Sharma, 1992. Surface active compounds from microorganisms. Bio/Technology., 10, 60-65 https://doi.org/10.1038/nbt0192-60
  6. Jae-Young Cha, Hae-Sun Kim, Young-Su Cho, Young- Choon Lee and Yong-Lark Choi., 2000. Characterization of Crude Oil Degradation by Klebsiella sp. KCL Isolated from Sea Water, Korean Journal of Life Science, 10(3), 300-306
  7. Jin-Hyeuk Kwon, Soo-Woong Kang, Kyung-Ae Son and Chang-Seuk Park., 2000, Gray Mold of Safflower Caused by Botrytis cinerea. The Korean Journal of Mycology, 28(1), 46- 48
  8. Joel Chopineau, Frank D. McCafferty, Michal Therlsod, and Alexander M. Kllbanov., 1988. Production of Biosurfactants from Sugar Alcohols and Vegetable Oils Catalyzed by Lipase in a Nonaqueous Medium. Biotechnology and Bioengineering, 31, 208-214 https://doi.org/10.1002/bit.260310305
  9. Kim Sun-Hee, Sang-Cheol Lee, Ju-Soon Yoo, Woo-Hong Joo, Soo-Yeol Chung and Yong-Lark Choi., 2004. Characterization of oil-degradation Biosurfactant produced by Bacillus sp. TBM40-3., J. Korean Soc. Appl. Biol. Chem., 47(2), 170-175
  10. K. S. M. Rahman, I. M. Banat, J. Thahira, Tha. Thayumanavan, P. Lakshmanaperrumalsamy., 1999. Bioremediation of gasolin contaminated soil by a bacterial consortium amended with poutry litter, coir pith and rhamnolipid biosurfactant., Bioresource Technology., 81, 25-32 https://doi.org/10.1016/S0960-8524(01)00105-5
  11. Michael C. Cirigliano and George M. Carman, 1984. Isolation of Bioemulsifier from Candida Lipolytica. Appl. Environ. Microbiol., 48(4), 747-750
  12. Michael C. Cirigliano and George M. Carman., 1985. Purification and Characterization of Liposan, a Bioemulsifier from Candida Lipolytica., Appl. Environ. Microbiol., 50(4), 846-850
  13. Nielsen TH, C Christophersen, U Anthoni, J Sorensen., 1999. Viscosinamide, a new cyclic depsipeptide with surfactant and antifungal properties produced by Psedononas fluorescens DR54. J Appl Microbiol 87(1):80-90 https://doi.org/10.1046/j.1365-2672.1999.00798.x
  14. Sang-Cheol Lee, Youn-Ju Jung, Ju-Soon Yoo, Young-Su Cho, In-Ho Cha and Yong-Lark Choi., 2002, Characteristic of Biosurfactants produced by Bacillus sp. LSC11., Korean Journal of Life Sicence, 12(6), 745-751
  15. Shaw, A., 1994. Surfactants-94., Soap Cosmet. Chem. Specialities, 70, 24-34
  16. Sarah J. Macnaughton, John R. Stephen, Albert D. Venosa, Gregory A. Davis, Yun-Juan Chang, and David C. White., 1999, Microbial Population Changes during Bioremediation of an Experimental Oil Spill., Appl. Environ. Microbiol., 65(8), 3566-3574
  17. Seog-won Chang, Sung-Kee Kim, Eun-Sup Yi and Jin-Won Kim., 2001, Occurrence of Gray Mold Caused by Botrytis cinerea on Cryptotaenia japonica in Korea, The Korean Society of Mycology, 29(4), 227-229
  18. Singh P., and S. S. Cameotra, 2004. Potential applications of microbial surfactants in biomedical sciences. TRENDS in Biotechnology, 22(3), 142-146 https://doi.org/10.1016/j.tibtech.2004.01.010
  19. Singer, M. E., 1985. Microbial biosurfactants., Microbes Oil Recovery, 1, 19-38
  20. Zajic, J. E., H. Gignard, and D. F. Gerson, 1977, Properties and biodegradation of a bioemulsifier from Corneybacterium hydrocarboclastus., Biotechnol. Bioeng., 19, 1303-1320 https://doi.org/10.1002/bit.260190905

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