DOI QR코드

DOI QR Code

Antitumor activity of Bacillus subtilis SW-1 isolated from Jeotgal

젓갈에서 분리한 Bacillus subtilis SW-1의 항암활성

  • 박종기 (진주산업대학교 미생물공학과) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 최영우 (신라대학교 식품영양학과) ;
  • 정영기 (동의대학교 미생물학과) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Published : 2004.10.01

Abstract

A bacterum containing antitumor activity was isolated from traditional korean food, Jeotgal. Through the 16s rRNA sequence analysis, the bacterium was identitied as a strain of Bacillus subtilis SW-l. The best culture condition for antitumor activity of the bacterium is 3% of soluble starch and 1 % of yeast extract as corbon and nitrogen sources, respectively. Cytotoxicitic concentrations of the culture supernatant of B. subtilis SW-1 against cancer cell lines, A549 and SK-OV3 were 30 ul/ml and 40 ul/ml, respectively, as $IC_{50}$/ values. In DNA fragmentation assay, the culture supernatant showed the programmed cell death (apoptosis) to cause degrading the chromosomal DNA like ladder. Taken together, the culture supernatant of the B. subtilis SW-1 has some possibility to be used as an antitumor agent.

본 연구에서 미생물유래의 종양 예방 및 억제제 개발을 목적으로 우리나라 전통 발효식품인 젓갈에서 항암 활성이 우수한 미생물을 분리, 동정 하였다. 젓갈로부터 항암물질을 생산하는 균주 SW-1을 분리하여 형태학적, 생리학적 특성을 검토한 결과, 본 균주는 Bacillus subtillis SW-1으로 동정되었다. 본 균주로부터 항암물질은 실험에 사용한 두가지의 암세포에 강한 활성을 나타내었다. 그 중에서 폐암 세포인 A549에 가장 강한 활성을 나타내었다. 항암물질 생산을 위한 최적 배양조건을 검토한 결과, 3.0% soluble starch, 1.0% yeast extract 였다. 무기염의 경우는 활성에는 그다지 영향을 미치지 않았다. 배지의 초기 pH 및 배양온도에 따른 항암물질의 생산성을 검토한 결과 초기 pH 5.0배양온도 3$0^{\circ}C$에서 25시 간배양 했을 때 최대 활성을 보였다. Chromosomal DNA단편화 실험에서 Bacillus subtilis SW-1의 배양상등액을 처리했을 때 DNA가 사닥다리모양으로 분해되는 Apoptosis(계획된 세포죽임)를 일으키는 것으로 확인되었다. 이 미생물이 생성하는 물질을 현재 HPLC, GC등을 이용하여 분리 중에 있으며 현재까지의 결과로는 당 중합체 인 것으로 분석된다.

Keywords

References

  1. Azuma, I., M. Yamawaki, T. Ogura, T. Youshimoto, R. Tokuzen, F. Hirao. and Y. Yamamura. 1978. Antitumor activity of BCG cell-wall skeleton and related materials. Gann. 21, 73-86
  2. Bogdanov, I. G., P. Popkhistov. and L. Marinov. 1962. Anticancer effect of antibioticum bulgaricum on Sarcoma- 180 and the solid from of Ehlich carcinoma. Abstr. VIII Intl. Cancer Congress. Moscow. Pp: 364-365
  3. Doll. R., R. Peto, 1981. The causes of cancer: quantitative estimate of avoidable risks of cancer in the United States today. J Natl Cancer Inst. 66, 1191-1308
  4. Friend., B. A. and K. M. Shahani. 1984. Antitumor properties of Lactobacilli and dairy products fermented by Lactobacilli. J. Food Prot. 47, 717-723
  5. Hrvoie, B., Z. Mirzet, D. Nullin. and F. I. Robin. 1993. Nuclear diacylglycerol is increased during cell proliferation in vivo. Biochem J. 290, 633-636
  6. Kato. I., S. Kobayaahi, T. Yokokura. and M. Mutai. 1981. Antitumor activity of Lactobacillus casei in mice. Gann. 72, 517-523
  7. Kim., E. R, B. M. Jung, J. Y. Kim, S. Y. Kim, H. K. Jung, H. J. Lee. and H. N. Chun. 2003. Basic physiological activities of Bifidobacterium infantis Maeil-K9 and lactobacillus plantarum KCTC3099 selected by anticarcinogenic activities. kor. J. Microbiol. Biotechnol. Vol. 31, No. 4, 348-354
  8. Kim, H. Y., H. S. Bae. and Y. J. Baek. 1991. Antitumor Activity of Lactobacterium against in vivo sarcoma 180 and Lewis Lung carcinoma. Korean Association of Immunobiologists. 23, 188-196
  9. Maron, J., W. C. Roberts, J. E. Edwards, H. A. McAllister, Foley and Stephen E. Epstein. 1978. Sudden death in patients with hypertrophic cardiomyopathy: Characterization of 26 patients without functional limitation. The American Journal of Cardiology. Vol. 41, Issue 5, 1, 803-810 https://doi.org/10.1016/0002-9149(78)90717-8
  10. Michael, C. A., A. S. Dominic. and M. Anue. 1988. Feasibility of drug screening with panels of human tumor cell line using a microculture tertrazolium assay. Cancer Res. 48, 589-596
  11. Milas, L. and M. T. Scott. 1978. Antitumor activity of Corynebacterium parvum, Adv, Cancer Res. 26, 257-306 https://doi.org/10.1016/S0065-230X(08)60090-1
  12. Miyazak, T., M. Nishiima. 1981, Structual examination of a water soluble antitumor polysaccharide of Ganoderma Iucidum. Chem Pharm Bull. 29, 3611-3616 https://doi.org/10.1248/cpb.29.3611
  13. Oh., C. Y. and W. K. Lee. 2000. Antitumor activity of lactic acid bacteria isolated from human intestine against sarcoma 180 in mice. Korean J. Lab. Anin. Sci. 16(4). 237- 244
  14. Richman, S. P., J. U. Gutterman. and E. E. Ribi. 1978. Phase I-Ⅱ study of intratumor immunotherapy with BCG cell wall skeleton plus P3 Cancer Immunol. Immunoter. 5, 41-44
  15. Shin, K. S., O. W. Chae, I. C. Park, S. K. Hong. and T. B. Choe. 1998. Antitumor effects of mice fed with cell lysate of Lactobacillus plantarum isolated from kimchi. Korean J. Biotechnol. Bioeng. Vol. 13, No 4, 357-363
  16. Waymouth, C. 1974. To disaggregate or not to diaggregate: Injury and cell diaggregation. transient or permanent. In Vitro, 10, 97-111 https://doi.org/10.1007/BF02615343
  17. Weisburger, J. H. 1996. On the etiology of gastro-intestinal tract cancer, with emphasis on dietary factary factors in Emmelot, Krick. Environmental Press. Amsterdam. 215- 240
  18. Yamaue, H., H. Tanimura, M. Iwahashi, M. Tani, T. Tsunoda, K. Tabuse, K. Kuribayashi. and K. Saitro. 1989. Role of interleukin-2 and interferon-r in induction of activated natural killer cells from mice primed in vivo and subsequently challenged in vitro with the Streptococcal preparation OK432. Cancer Immunol. Immunother. 29, 79-86 https://doi.org/10.1007/BF00199281

Cited by

  1. Antioxidant Activity of Diketopiperazines from Bacillus idriensis vol.59, pp.6, 2015, https://doi.org/10.5012/jkcs.2015.59.6.545
  2. Antioxidant Activity of a Chitin-degrading Bacterium Bacillus idriensis (CGH18) vol.28, pp.4, 2013, https://doi.org/10.7841/ksbbj.2013.28.4.217