Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Cytotoxicity and DNA Topoisomerases Inhibitory Activity of Constituents from the Sclerotium of Poria cocos

  • Li, Gao (College of Pharmacy, Yanbian University) ;
  • Xu, Ming-Lu (College of Pharmacy, Yeungnam University) ;
  • Lee, Chong-Soon (Department of Biochemistry, College of Science, Yeungnam University) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu) ;
  • Chang, Hyun-Wook (College of Pharmacy, Yeungnam University) ;
  • Son, Jong-Keun (College of Pharmacy, Yeungnam University)
  • Published : 2004.08.01
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Abstract

The bioactivity-guided fractionation of the methylene chloride extract of the sclerotium of Poria cocos led to the isolation of (S)-(＋)-turmerone (1), ergosterol peroxide (2), polyporenic acid C (3), dehydropachymic acid (4), pachymic acid (5), and tumulosic acid (6). Compounds 4-6 exhibited moderate cytotoxicities, with $IC_{50}$ values of 20.5, 29.1, and $10.4{\;}\mu\textrm{m}$, respectively, against a human colon carcinoma cell line. However, 3-6 not only showed inhibitory activities as potent as etoposide used as a positive control on DNA topoisomerase II (36.1, 36.2, 43.9 and 66.7％ inhibition at a concentration of $20{\;}\mu\textrm{m}$, respectively), but also inhibition of DNA topoisomerase I (55.8, 60.7, 43.5, and 83.3％ inhibition at a concentration of $100{\;}\mu\textrm{m}$, respec-tively).

Keywords

References

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