YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Determination of Hyperin in Acanthopanax senticosus and A. sessiliflorus by HPLC

HPLC에 의한 가시오가피 및 오가피 중 Hyperin의 정량

  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

A new high performance liquid chromatographic method was applied for the determination of hyperin in Acanthopanax senticosus and A sessiliflorus. The stationary phase used was $\beta$-Bondapak $C_{18}$ reverse-phase column and a mobile phase program was a gradient of acetonitrile and distilled water at a flow rate of 1.0 ml/min. Hyperin was detected at 210 nm, and the analysis was successfully carried out within 20 min. Hyperin was detected in the one year-grown and two years-grown stem of A senticosus (0.47 and 0.13 mg/g, respectively) and A sessiliflorus (0.14 and 0.03 mg/g, respectively). Hyperin was detected in the main and branch root of A sessiliflorus (0.30 and 0.09 mg/g, respectively). But there is no detection of hyperin in the main and branch root of A senticosus.

Keywords

References

  1. Yook, C. S., Lee, D. H. and Seo, Y. K. : A new forma of Acanthopanax species (I). Kor. J. Pharmacogn. 7, 179 (1976)
  2. Lee, S., Jung, S. H., Lee, Y. S. and Shin, K. H. : Hyperin, an aldose reductase inhibitor from Acanthopanax senticosus leaves. Nat. Prod. Sci. 9, 4 (2003)
  3. Kim, J. H. and Yang, K. S. : Antilipid peroxidative effect of Houttuynia cordata. Yakhak Hoeji 45, 494 (2001)
  4. Lee, M. H., Son, Y. K. and Han, Y. N. : Tissue factor inhibitory flavonoids from the fruits of Chaenomeles sinensis, Arch. Pharm. Res. 25, 842 (2002) https://doi.org/10.1007/BF02977002
  5. Lee, S., Shin, D. S., Oh, K. B. and Shin, K. H. : Antibacterial compounds from the leaves of Acanthopanax senticosus. Arch. Pharm. Res. 26, 40 (2003)
  6. Chen, M., Song, F, Guo, M., Liu, Z. and Liu, S. : Analysis of flavonoid constituents from leaves of Acanthopanax senticosus Harms by electrospray tandem mass spectrometry. Rapid Commun. Mass Spectrom. 16, 264 (2002) https://doi.org/10.1002/rcm.574
  7. Sha, M., Cao, A. and Yang, S. : Determination of hyperin in Epimedium koreanum Nakai by HPLC. China J. Chin. Materia Medica 20, 357 (1995)
  8. Wu, Y., Zhou, S. D. and Li, P. : Determination of flavonoids in Hypericum, Hypericum perforatum by HPLC analysis. Acta Pharmaceut. Sin. 37, 280 (2002)
  9. Sha, M., Cao, A., Wang, B., Liu, C., Geng, J. and Liu, W. : Determination of hyperin in Sanguisorba officialis L. by high performance liquid chromatography. Chin. J. Chromatogr. 16, 226 (1998)
  10. Chinnici, F., Gaiani, A., Natali, N., Riponi, C. and Galassi, S. : Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column. J. Agric. Food Chem. 52, 3 (2004)
  11. Lommen, A., Godejohann, M., Venema, D. P., Hollman, P. C. and Spraul, M. : Application of directly coupled HPLC-NMR-MS to the identification and confirmation of quercetin glycosides and phloretin glycosides in apple peel. Anal. Chem. 72, 1793 (2000) https://doi.org/10.1021/ac9912303
  12. Kang, S. S., Um, B. H., Kim, J. S. and Ahn, B. T. : Isolation of flavonoids from Evodiae Fructus. Kor. J. Pharmacgn . 28, 9 (1997)
  13. Lee, S., Kim, B. K., Cho, S. H. and Shin, K. H. : Phytochemical constituents from the fruits of Acanthopanax sessiliflorus.Arch. Pharm. Res. 25, 280 (2002) https://doi.org/10.1007/BF02976626