Functional Analysis of Protein Chip Plate Using Silane Carboxylate Surface

실란 카르복실 표면을 사용한 단백질 칩 기판의 기능 분석

  • 김지현 (단국대학교 자연과학대학 자연과학부) ;
  • 송예신 (단국대학교 자연과학대학 자연과학부) ;
  • 윤미영 (단국대학교 자연과학대학 자연과학부) ;
  • 피재호 (단국대학교 자연과학대학 자연과학부)
  • Published : 2004.08.01

Abstract

We fabricated protein chip plates coated with silane carboxylate. The silane compound was immobilized by hydrogen bond and/or other chemical bonds on the surface of the plate. The plates were then prepared by binding $Ni^{2+}$ to surfaces terminated with silane carboxylate groups. The carboxylic acid surface was generated by chemical oxidation of the terminal double-bond functions of the silane-deposited layer. The $Ni^{2+}$ ions on the surface reacted readily to His-tagged proteins. A significant increase in His-tagged protein adsorption was achieved on the surface terminated with silane carboxylate with longer alkyl chain, suggesting better availability of these protein chip plates for proteomic studies.

Keywords

References

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