Biomedical Science Letters (대한의생명과학회지)
- Volume 10 Issue 2
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- Pages.75-84
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- 2004
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
DNA Chip using Single Stranded Large Circular DNA: Low Background and Stronger Signal Intensity
- Park, Jong-Gu (Department of Medical Genetic Engineering, Keimyung University School of Medicine Dongsan Medical Center, WelGENE Inc.)
- Published : 2004.06.01
Abstract
Massive identification of differentially expressed patterns has been used as a tool to detect genes that are involved in disease related process. We employed circular single stranded sense molecules as probe DNA for a DNA chip. The circular single stranded DNAs derived from 1,152 unigene cDNA clones were purified in a high throughput mode from the culture supernatant of bacterial transformants containing recombinant phagemids and arrayed onto silanized slide glasses. The DNA chip was examined for its utility in detection of differential expression profile by using cDNA hybridization. Hybridization of the single stranded probe DNA were performed with Cy3- or Cy5-labeled target cDNA preparations at
Keywords
- DNA chip;
- Large circular (LC-) sense molecule;
- Probe DNA;
- Differential gene expression;
- Recombinant phagemids