Journal of Microbiology

  • 제42권2호
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  • Pages.80-86
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  • 2004
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Molecular Investigation of Two Consecutive Nosocomial Clusters of Candida tropicalis Candiduria Using Pulsed-Field Gel Electrophoresis

  • Park, Joon (Departments of Urology, Chosun University College of Medicine) ;
  • Shin, Jong-Hee (Departments of Laboratory Medicine, Chonnam National University Medical Schoo) ;
  • Song, Jeong-Won (Departments of Laboratory Medicine, Chonnam National University Medical Schoo) ;
  • Park, Mi-Ra (Departments of Laboratory Medicine, Chonnam National University Medical Schoo) ;
  • Kee, Seung-Jung (Departments of Laboratory Medicine, Chonnam National University Medical Schoo) ;
  • Jang, Sook-Jin (Laboratory Medicine, Chosun University College of Medicin) ;
  • Park, Young-Kyu (General Surgery, Chonnam National University Medical Schoo) ;
  • Suh, Soon-Pal (Departments of Laboratory Medicine, Chonnam National University of Medical Schoo) ;
  • Ryang, Dong-Wook (Departments of Laboratory Medicine, Chonnam National University Medical School)
  • 발행 : 2004.06.01
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초록

Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 21 Candida tropicalis isolates collected from urine specimens of 11 patients and one healthcare worker, in an intensive care unit (ICU) over a 4-month period. Seventeen epidemiologically unrelated strains from 14 patients were also tested to determine the discriminatory power of PFGE. PFGE typing consisted of electrophoretic karyotyping (EK) and restriction endonuclease analysis of genomic DNA (REAG), using two restriction enzymes (BssHII and SfiI). The EK pattern was the same in all 38 isolates, while REAG using SfiI separated the isolates into nine types. However, 16 different PFGE types were iden-tified by REAG with BssHII, and the same results were obtained when the results of both REAG tests were combined. In serial urinary isolates from 10 patients, all strains from each patient had the same PFGE pattern. While the epidemiologically unrelated strains from 14 patients consisted of 13 different PFGE types, the 20 isolates from the 11 ICU patients fell into only two PFGE types (types Cl and C2), and these apparently originated from the two different outbreaks. All strains of type Cl (n = 12) were isolated from six patients, between November 1999 and January 2000, and all of the type C2 strains (n=8) were isolated from five patients, during January and February 2000. This study shows two con-secutive clusters of C. tropicalis candiduria in an ICU, defined by PFGE typing, and also demonstrates that a PFGE typing method using BssHII is perhaps the most useful method for investigating C. tropi-calis candiduria.

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