Biotechnology and Bioprocess Engineering:BBE

  • 제9권2호
  • /
  • Pages.107-111
  • /
  • 2004
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Bioelectrocatalyzed Signal Amplification for Affinity Interactions at Chemically Modified Electrodes

  • Hyun C. Yoon (Department of Biotechnology, Ajou University) ;
  • Kim, Hak-Sung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • 발행 : 2004.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A comparative study was performed to evaluate the signal amplification strategies in electrochemical affinity sensing, which included the direct electron transfer and diffusible-group mediated electron transfer between label enzymes that were specifically bound to target proteins and chemically modified electrode surfaces. As a platform surface for affinity recognition reactions, a double functionalized poly(amidoamine) dendrimer monolayer that was modified with ferrocene and biotin groups was constructed on a gold surface. With the chemically modified electrode, a model affinity sensing with avidin was investigated. The advantages of adopting the diffusible-group mediated signaling strategy were demonstrated in terms of signal sensitivity and stability.

키워드

참고문헌

  1. Ann. Rev. Biomed. Eng. v.1 Microfabrication in biology and medicine Voldman,J.;M.L.Gray;M.A.Schmidt https://doi.org/10.1146/annurev.bioeng.1.1.401
  2. Lab Chip v.1 Trends in miniaturized total analysis systems for point-of-care testing in clinical chemistry Tudos,A.J.;G.A.J.Besselink;R.B.M.Schasfoort https://doi.org/10.1039/b106958f
  3. Curr. Opin. Chem. Biol. v.6 Miniaturised multiplexed immunoassays Joos,T.O.;D.Stoll;M.F.Templin https://doi.org/10.1016/S1367-5931(01)00289-7
  4. Biotechnol. Bioprocess Eng. v.8 Miniaturization of polymerase chain reaction Lee,J.Y.;J.J.Kim;T.H.Park https://doi.org/10.1007/BF02942268
  5. Anal. Chem. v.73 Electric manipulation of bioparticles and macromolecules on microfabricated electrodes Huang,Y.;K.L.Ewalt;M.Tirado;R.Haigis;A.Forster;D.Ackley;M.J.Heller;J.P.O'Connell;M.Krihak https://doi.org/10.1021/ac001109s
  6. Biotechnol. Bioprocess Eng. v.8 Nanoparticle-based detection technology for DNA analysis Park,H.G. https://doi.org/10.1007/BF02942269
  7. Methods v.22 Development of rapid one-step immunochromatographic assay Paek,S.H.;S.H.Lee;J.H.Cho;Y.S.Kim https://doi.org/10.1006/meth.2000.1036
  8. J. Immunol. Methods v.279 Development of a quantitative lateral-flow assay for rapid detection of fatty acid-binding protein Chan,C.P.Y.;K.W.Sum;K.W.Cheung;J.F.C.Glatz;J.E.Sanderson;A.Hempel;M.Lehmann;I.Renneberg;R.Renneberg https://doi.org/10.1016/S0022-1759(03)00243-6
  9. Electrophoresis v.23 Microchip-based amperometric immunoassays using redox tracers Wang,J.;A.Ibanez;M.P.Chatrathi https://doi.org/10.1002/1522-2683(200211)23:21<3744::AID-ELPS3744>3.0.CO;2-B
  10. Anal. Chem. v.75 Electrochemical protein chip with arrayed immunosensors with antibodies immobilized in a plasma-polymerized film Kojima,K.;A.Hiratsuka;H.Suzuki;K.Yano;K.Ikebukuro;I.Karube https://doi.org/10.1021/ac0257391
  11. Lab Chip v.3 A polymer-based microfluidic device for immunosensing biochips Ko,J.S.;H.C.Yoon;H.Yang;H.B.Pyo;K.H.Chung;S.J.Kim;Y.T.Kim https://doi.org/10.1039/b301794j
  12. Anal. Biochem. v.282 Affinity biosensor for avidin using a double functionalized dendrimer monolayer on a gold electrode Yoon,H.C.;M.Y.Hong;H.S.Kim https://doi.org/10.1006/abio.2000.4608
  13. Langmuir v.17 Reversible association/dissociation reaction of avidin on the dendrimer monolayer functionalized with a biotin-analog for a regenerable affinity-sensing surface Yoon,H.C.;M.Y.Hong;H.S.Kim https://doi.org/10.1021/la001373g
  14. Bull. Kor. Chem. Soc. v.24 Patterning biological molecules onto poly(amidoamine) dendrimer on gold and glass Hong,M.Y.;D.Lee;H.C.Yoon;H.S.Kim https://doi.org/10.5012/bkcs.2003.24.8.1197
  15. J. Am. Chem. Soc. v.124 Electron-transfer mechanisms through biological redox chains in multicenter enzymes Jeuken,L.J.;A.K.Jones;S.K.Chapman;G.Cecchini;F.A.Armstrong https://doi.org/10.1021/ja012638w
  16. J. Am. Chem. Soc. v.125 Long-range electron transfer over 4 nm governed by an inelastic hopping mechanism in self-assembled monolayers of helical peptides Morita,T.;S.Kimura https://doi.org/10.1021/ja034872n
  17. J. Am. Chem. Soc. v.120 Electron transfer in organized assemblies of biomolecules. Construction and dynamics of avidin/biotin co-immobilized glucose oxidase/ferrocene monolayer carbon electrodes Anicet,N.;A.Anne;J.Moiroux;J.M.Saveant https://doi.org/10.1021/ja9803097
  18. Biosens. Bioelectron. v.17 Differentiating the orientations of photosynthetic reaction centers on Au electrodes linked by different bifunctional reagents Zhao,J.;Y.Zou;B.Liu;C.Xu;J.Kong https://doi.org/10.1016/S0956-5663(02)00026-X
  19. J. Phys. Chem. B v.102 Electron transfer in organized assemblies of biomolecules. Step-by-step avidin/biotin construction and dynamic characteristics of a spatially ordered multilayer enzyme electrode Anicet,N.;C.Bourdillon;J.Moiroux;J.M.Saveant https://doi.org/10.1021/jp982352m