Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Ethylacetate Fraction of Plantain (Plantago asiatica L.) on Experimentally-Induced Gastric Mucosal Damage and Gastric Ulcers in Rats

질경이가 실험적으로 유발된 흰쥐의 위염 및 위궤양에 미치는 영향

  • 원영준 (조선대학교 식품영양학과) ;
  • 나명순 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2004.04.01
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Abstract

Plantain has been used for antidiarrhea, antihemorrhage and the remedy of indigestion. Plantain was extracted with ethanol and fractionated systemically with n-hexane, chloroform, ethylacetate (EtOAC) and n-butanol. Antioxidant index (AI was expressed as induction period of oil containing various fractions/induction period of oil of 600 ppm) of EtOAC fraction was the highest among fractions in vitro. The protective effects of the EtOAC fraction of plantain (PE) administered 1 mL orally or intraduodenally on experimentally induced gastritis, gastric ulcer and gastric secretion were evaluated in rats. Sprague-Dawley rats weighing 250∼300 g were divided into 4 groups; negative control group (CON), PE 200 mg/kg treated group (PEL), PE 400 mg/kg treated group (PEH) and positive control group (cimetidine 100 mg/kg-CMT or omeprazol 100 mg/kg treated group-OMT), respectively, PE significantly suppressed HCl-ethanol induced gastric lesions and indomethacin-induced gastric ulcers (administered subcutaneouly) in rats. Specially PE 400 mg/kg showed significantly inhibitory effect, which was more potent than that of 100 mg/kg of commercial drug, cimetidine, and elevated an inhibitory effect to be close to the level in inhibitory ratio of omeprazol administered group in Shay's ucler. On gastric secretion in pylorus ligated rat, PE 200 mg/kg and 400 mg/kg decreased the gastric volume and acid output, but did not show an apparent effect on pepsin activity. In addition, PE 400 mg/kg depressed gastric ulcers induced by water immersion stress and duodenal ulcers induced by cysteamine administered subcutaneouly. These results suggest that the ethylacetate fraction of plantain can be used in prevention and treatment of experimentally induced gastric mucosal damage and ulcers.

본 실험에서는 흰쥐에서 실험적으로 급성위염, 위궤양 및 십이지장궤양을 유발하고 질경이 에칠아세테이트분획을 투여하여 항위염 및 항위궤양작용에 미치는 영향을 검토하였던 바 다음과 같은 결과를 얻었다. 1. 질경이 에탄올추출물을 n-hexane, chloroform, ethylactae 및 n-butanol로 계통분획하고 농축하여 in vitro에서 항산화력을 측정한 결과 ethyl-acetate분획이 가장 우수하였다. 2. 질경이 에칠아세테이트분획 (PEL 및 PEH)은 HCl-ethanol에 의한 위점막 손상에서 대조군(CON)에 비하여 각 용량별로 55.7% 및 62.1%의 유의적인 항위염작용을 나타냈으며, 특히 고용량투여군인 PEH의 경우 양성 대조약물투여군(60.5%)보다 우수한 효과를 나타냈다. 3. Indomethacin에 의해 유발된 위궤양에서, PEL 및 PEH는 양성 대조약물군인 CMT의 위궤양 억제율(61.6%)에는 다소 미치지 못하였으나 CON에 비하여 유의성 있는 억제 효과를 나타냈다. 4. Shay위궤양에서, 질경이 에칠아세테이트분획 200mg/kg을 십이지장에서 위내로 투여하였을 때 궤양억제율이 37.9%로 CON에 비하여 Shay위궤양을 억제하였으나 유의성있는 효과는 아니었으며 용량을 증량하여 투여한 PEH(400 mg/kg)는 48.9%로 양성 대조약물인 omeprazol를 투여한 OMP의 저해효과(55.6 %)에 근접하였다. 5. 질경이의 에틸아세테이트분획을 용량별로 투여했을 때 위액의 pH가 대조군에 비해 약간의 감소되었으나 정상 범위 내로 유의성은 인정되지 않았고 양성 대조약물군인 OMP는 유의적으로 위액의 pH를 유의성있게 감소시켰다. 위액량 및 총 위산분비량의 경우, 대조군에 비하여 PEL 및 PEH가 각각 33.8%와 38.7%의 감소효과를 나타냈으나 유의성은 없었으며 양성 대조약물인 omeprazol 만큼 뚜렷한 효과는 볼 수 없었다. 6. Pepsin활성에서, 질경이 에틸아세테이트분획과 양성 대조약물투여로 pepsin 활성이 대조군에 비해 각각 약 17%, 22% 및 24%의 활성저해 효과를 나타냈으나 전군에서 유의성 있는 변화는 아니었다. 7 구속ㆍ수침스트레스에 의해 유발된 궤양의 경우, PEL 및 PEH에서도 출혈을 동반한 궤양이 관찰되었으나 CON에 비하여 궤양의 깊이가 다소 얕았지만 유의적인 효과는 아니었다. 양성 약물대조군의 경우 궤양의 형성이 CON과 PEL및 PEH에 비하여 궤양형성의 부분이 다소 적었으나 점상 출혈이 존재하였다. 8. Cysteamine에 의해 유발된 십이지장궤양에서, CMT가 52.2%의 유의한 궤양 억제율을 나타냈고 PEH의 경우 유의성은 없었으나 약 43%의 궤양억제율을 나타냈다.

Keywords

References

  1. Sucroalfate Pathology of peptic ulcer disease Halter,F.;Hollander,D.(eds.);Tygat,G.N.J.(eds.)
  2. Yakhak Heoeji v.27 Evaluation of neutralizing capacities of antacid products Park,K.H.;Cha,S.M.;Choi,J.S.;Ki,N.D.
  3. Free Rad Res Comm v.7 Role of oxygenderived free radicals in gastric mucosal injury induced by ischemia or ischemia-reperfusion in rats Yoshikawa,T.;Ueda,S.;Naito,Y.;Takahashi,S.;Oyamada,H.;Morita,Y.;Yoneta,T.;Kondo,M. https://doi.org/10.3109/10715768909087953
  4. Planta Med v.45 Inhibition of mast cells hitamine release by flavonoids and bioflavonoids Amellal,M.;Bronner,C.;Briancon,F.;Haag,M.;Anton,R.;Landry,Y.
  5. Biochem Pharmacol v.43 Effects of flavonoids on immune response and inflamatory cell funcitions Middleton,E.;Kanadaswami,C. https://doi.org/10.1016/0006-2952(92)90489-6
  6. Biochem Biophys Res Commum v.157 Effects of genistein on topoisomerase activity and on the growth of Ha-ras-transformed NIH 3T3 cells Okura,A.;Arakawa,H.;Yoshinari,T.;Monden,Y. https://doi.org/10.1016/S0006-291X(88)80030-5
  7. Digestion v.43 Role of oxygen-derived free radicals in the mechanism of chronic gastric ucerlation in the rat: Implications for cytoprotection Salim,A.S. https://doi.org/10.1159/000199868
  8. Dig Dis v.23 Stimulation of mucus and non-parietal cell secretion by E₂prostaglandins Boltion,J.P.;Palmer,D.;Cohen,M.M. https://doi.org/10.1007/BF01072421
  9. Unesco Regional Seminar on the Chemistry, Pharmacology and clinical use of flavonoid compounds The effect of Artemisia Herba on gastric lesion and ulcers in rats with isolation of eupatilin Lee,E.B.
  10. MS Thesis. Choongbuk National University A mechanism of gastric antiulceration ny the extract of Artemisiae argyi Lee,J.S.
  11. MS Thesis. Chosun University Effect of Sophorae Radix methanol Ex. on the experimentaly induced gastric ulcer in rats Oh,J.
  12. PhD Thesis. Duksung University A study on the antigastric and antiulcer effects of propolis extract and its effective compounds Kim,E.J.
  13. MS Thesis. Duksung University The antigastric and antiulcer action og the extract of panax ginseng Radix Song,Y.O.
  14. Korean folk medicine Lee,S.J.
  15. The encyclopedia of medicinal plants Park,J.H.;Lee,J.K.
  16. MS Thesis. Korea University A taxonomic and systematic study of Genus Pantago in Korea Park,C.H.
  17. Jpn J Pharmacol v.33 Effect of polyamines on acidified ethanol-induced gastic lesion in rats Mizui,T.;Doteuchi,M. https://doi.org/10.1254/jjp.33.939
  18. Clinical Phamaco v.113 Effects of meta-magnesium aluminosillicate. Extract of Licirice root and their combination on various experimental gastric ulcers Ueno,N.;Suzuki;Okabe,S.
  19. Japan J Pharmacol v.27 Effects of various amino acids on indomethacin-induced gastric ulcers in rats Urushidani,T.;Okabe,S.;Takeuchi,K.;Takagi,K. https://doi.org/10.1254/jjp.27.316
  20. Gastroenterology v.5 The experimental gastric ulcers in rats Shay,H.;Kpmarrov,S.A.;Fels,S.S.
  21. Gastroenterol v.5 A simple method for the uniform production of gastric ulceration in the rat Shay,H.;Kormarov,S.A.;Fels,S.S.;Meramze,D.;Gruenstein,M.;Siplet,H.
  22. Gastroenterology v.83 Effect of substituted benzimidazole (H 149/94) on gastric acid secretion in humans Olbe,L.;Haglund,U.;Leth,R.;Lind,T.;Cedergerg,C.;Ekenved,G.;Elander,B.;Fellenius,E.;Lunborg,P.;Wallmark,B.
  23. J Gen Physio v.22 The estimation of pepsin, papain and catherpsin with hemoglobin Anson,M.L. https://doi.org/10.1085/jgp.22.1.79
  24. Kor J Food Hygiene v.5 The study of new model for stree-induced gastric ulcer in rats Lim,Y.K.;Lee,J.K.;Lee,Y.S.
  25. Gastroenterology v.77 Effects of water-immersion stress on gastric secretion and mucosal blood flow in rats Kitagwa,H.;Fujiwara,M.;Osumi,Y.
  26. Japan J Pharmacol v.18 The effect of drugs on the production and recovery process of the stress ulcer Takagi,K.;Okabe,S. https://doi.org/10.1254/jjp.18.9
  27. Dig Dis Sci v.31 Gastric acid secretion and lesion formation in rats under water-immersion stress Hayase,M.;Takeuchi,K. https://doi.org/10.1007/BF01300703
  28. Gastroenterology v.88 Cysteamine and propionitrile inhinit the rise of duodenal mucusal alkine secretion in respose to luminal acid in rats Briden,S.;Flemstroim,G.;Kivilaaakso,E. https://doi.org/10.1016/S0016-5085(85)80183-9
  29. Gastroenterology v.98 MPTP-induced duodenal ulcers in rat Keshavarzian,A.;Wibowo,A.;Gordonn,J.H.;Fields,J.Z.
  30. Eur J Pharmacol v.36 Gastric acid stimulating action of cysteamine in the rat Ishii,T.;Fujii,Y.;Homma,M. https://doi.org/10.1016/0014-2999(76)90086-8
  31. Gastroenterology v.77 Cytoprotection by prostaglansins in rats. Prevention of gastric mecrosis produced by alcohol, HCL, NaOH, hypertonic NaCl and thermal injury Robert,A.;Zamins,J.E.;Lan caster,C.;Hancher,A.J.
  32. Japan J Pharmacol v.46 Cytoprotective action of cetrexate against HCL-ethanol induced gastric lesion in rats Kurebatashi,Y.;Ikeda,T.;Osada,Y. https://doi.org/10.1254/jjp.46.17
  33. Surg Forum v.30 Mechanism of cytoprotective effects of PGE₂ Vahi,R.;Guttman,F.M.;Mimaker,B.
  34. J Pharm Pharmacil v.43 Protective effect of cimetidine on tannic acid-induced gastric damage in rats Hung,C.R.;Lee,C.H. https://doi.org/10.1111/j.2042-7158.1991.tb03536.x
  35. N Engl J Med v.293 Inhibition of gastric acid secretion by cimetidine in patients with duodenal ulcer Henn,R.M.;Isenberg,J.I.;Maxwell,V.;Sturdevant,R.A.L.
  36. Gut v.17 Inhibition of food stimulated gastric acid secretion by cimetidine Pounder,R.E.;Williams,J.G.;Russell,R.E.C. https://doi.org/10.1136/gut.17.3.161
  37. Gastroenterol v.4 Effects of cimetidine on interinsic factor and pepsin secrestion in man Binder,H.J.;Donaldson,R.M.
  38. Am J Physiol v.243 Importentance of food in the regulation of gastrin release and formation Lichtenberger,L.M.
  39. Gastroenterology v.94 Studies on the mechanism of ethanol-induced gastric damage in rats Oates,P.J.;Hakkinen,J.P
  40. J Pharm Pharmacol v.45 Role of polymorphonuclear leucocytes and oxygen derived free radicals in the formation of gastric lesions induced by HCL/EtOAC, and possible mechanism of protection by antiulcer poly saccharide Mattsmoto,T.;Moriguchi,T.;Yamada,H. https://doi.org/10.1111/j.2042-7158.1993.tb05594.x
  41. Digestion v.49 Oxygen free radicals and lipid peroxidation in the pathogenesis of gastric mucosal lesions induced by indomethacin in rats Takeuchi,K.;Usehima,K.;Hironaka,Y.;Fujioka,Y.;Matsumoto,J.;Okabe,S. https://doi.org/10.1159/000200718
  42. Br J Pharmacol v.60 Mechnisms underlying gastric mucosal damage induced by indomethacin and bile-salts, and the actions of prostaglandin Whittle,B.J.R. https://doi.org/10.1111/j.1476-5381.1977.tb07522.x
  43. Am J Path v.54 Small intestinal ulcers nd intestinal flora in rats given indimethacin Kent,T.H.;Cardelli,R.M.;Stamier,F.W.
  44. Int J Clin Pharm Ther Toxicol v.23 Ulcer-healing drugs and endogeneous prostaglandin Guslandi,M.
  45. Scand J Gastroenterol v.19 Both topical and systemic treatments with 16, 16-dimethyl prostaglandin E2 are tropic to rat gastric mucosa Halter,F.
  46. Gastroenterology v.85 Influence of longterm 16, 16-dimethyl prostaglandin E2 treatment on the rat gastrointestinal mucusa Reinhart,W.H.;Muller,O.;Halter,F.
  47. Eur J Pharmacol v.40 Rela ionship between the prevention of rat gastric erosions and the inhibition of acid secretion by protaglandins Whittle,B.J.R. https://doi.org/10.1016/0014-2999(76)90057-1
  48. J Clin Gastroenterology v.12 The role of endogenous free radical scavenger on tissue recovery in the experimental ulcer model Oshima,A.;Asayama,K.;Sakai,N.;Kitajima,M. https://doi.org/10.1097/00004836-199001001-00011
  49. Scand J Gastroenterol v.20 Toxicological studies on omeprzol Ekman,L.;Hansson,E.;Havu,N.;Carlsson,E.;Lumdberg,C. https://doi.org/10.3109/00365528509095819
  50. Jpn J Pharmacol v.40 Effect of 15 (R)-15-methyl protaglandin E₂(Arbaprostil) on gastirc secretion and various gastric lesions induced in rats Okabe,S.;Jino,H.;Nishida,A.
  51. Am J Physiol v.205 Separation of pepsinogen Ⅰ, pepsinogen Ⅱ, and pepsinogen Ⅲ from human gastric mucosa Sciffers,M.J.;Segal,M.L.;Miller,L.L.
  52. Japan J Pharmacol v.34 Effect of anti-ulcer agent, 2'-arboxtmethoxy-4,4'-bis(3-metyly-2-butenyloxy) chalcone (SU-88), on the biosynthesis of gastric sulfted mucosubustances in restrained and water-immersed rats Suwa,T.;Fukushima,K.;Koyogoku,K. https://doi.org/10.1254/jjp.34.89
  53. Am J Physiol v.242 Gastroduodenal HCO₃transport characteristics and proposed role acidity regulation and mucosal protection Flemstrom,G.;Ganrner,A.
  54. Korea J Gastroenterology v.24 Influence of various factors on cysteamine -induced duodenal ulcers in the rats Hahm,K.B.;Lee,S.I.;Yim,D.S.;Kim,W.H.

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