Biotechnology and Bioprocess Engineering:BBE

  • 제9권1호
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  • Pages.62-64
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  • 2004
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Biocatalytic Preparation of Chiral Epichlorohydrins Using Recombinant Pichia pastoris Expressing Epoxide Hydrolase of Rhodotorula glutinis

  • Kim, Hee-Sook (Department of Food Science and Technology, Kyungsung University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University) ;
  • Park, Sunghoon (Department of Chemical and Biochemical Engineering, Pusan National University) ;
  • Lee, Eun-Yeol (Department of Food Science and Technology, Kyungsung University)
  • 발행 : 2004.01.01
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초록

The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinant Pichia pastoris with the enantioselective epoxide hydrolase of Rhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5%(v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100% ee and a yield of 26% were obtained within 5min from 50mM racemates.

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참고문헌

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