Biotechnology and Bioprocess Engineering:BBE

  • 제9권1호
  • /
  • Pages.41-46
  • /
  • 2004
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Production of Ginkgolides and Bilobalide from Optimized the Ginkgo biloba Cell Culture

  • Park, Young-Goo (Department of Forestry, Kyungpook National University) ;
  • Kim, Su-Jung (Department of Forestry, Kyungpook National University) ;
  • Kang, Young-Min (Department of Forest Science, Gyeongsang National University) ;
  • Jung, Hee-Young (Department of Forest Science, Gyeongsang National University) ;
  • D. Theertha Prasad (Department of Biotechnology, University of Agricultural Science, GKVK) ;
  • Kim, Sun-Won (Department of Food Nutrition, Gyeongsang National University) ;
  • Chung, Young-Gwan (Department of Forest Science, Gyeongsang National University) ;
  • Park, Myung-Suk (Department of Forest Science, Gyeongsang National University)
  • 발행 : 2004.01.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The influence of various culture conditions on growth and ginkgolides (GKA and GKB), and bilobalide formation in callus and suspension cultures of Ginkgo biloba were investigated. Callus induced from the leaf petioles exhibited distinct morphological and physiological responses. The cell biomass and ginkgolides content varied among the cell lines; brownish callus lines produced high levels of ginkgolides and bilobalide in spite of poor cell growth. Among the culture media used, MS medium showed significant effect on cell growth and ginkgolides production. Low concentration of sucrose (3%) improved cell growth, while higher sucrose levels (5 and 7%) improved ginkgolides production. Cultivation of callus cultures above 28$^{\circ}C$ dramatically reduced their growth rate; however the cell lines grown at 36$^{\circ}C$ showed increased levels of bilobalide content. A 2.5-L balloon type bubble bioreactor (BTBB) was successfully developed for the cell growth and ginkgolides production.

키워드

참고문헌

  1. J. Chromatogr. v.51 Determination of ginkgolides and bilobalide in Ginkgo biloba leaves and phytopharmaceuticals Van,B.A.;H.A.Scheeren;T.Rantio;W.C.Melger;G.P.Lelyveld https://doi.org/10.1016/S0021-9673(01)96886-4
  2. Plant Cell Culture: A Practical. Approach Callus formation by Ginkgo biloba embryos on hormone free media controlled by closures and media components Webb,D.;W.Arias;E.D.Hostos;R.A.Dixon(ed.)
  3. Plant Cell Culture: A Practical. Approach Secondary product formation by cell suspension cultures Morris,P.;A.H.Scragg;N.J.Smart;A.Stafford;R.A.Dixon(ed.)
  4. Plant Cell Culture: A Practical. Approach Changes in the amino acid composition of medium and cells of a plant tissue culture during growth in a liquid medium containing arginine Tulecke,W.;A.Rutner;R.A.Dixon(ed.)
  5. Nature v.203 A haploid tissue culture from the female gametophyte of Ginkgo biloba Tulecke,W. https://doi.org/10.1038/203094a0
  6. Plant Tissue Cell Culture Induction of embryogenesis in embryoderived callus of Ginkgo biloba (5nd ed.) Yates,W.
  7. International Congress of Plant Tissue and Cell Culture (2nd ed.) Carrier,D.J.;P.Conlombe;M.Mancini;R.Neufeld;M.Weber;J.Archambault
  8. Physiol. Plant. v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murasshige,T.;F.Skoog https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  9. Exp. Cell. Res. v.50 Nutrient requirements of suspension cultures of soybean root cells Gamberg,O.L.;R.A.Miller;K.Ojima
  10. Can. J. Bot. v.50 Medium and techniques for induction and growth of monocotyledonous plant cell culture Schemk,R.U.;A,C,Hildebrandt https://doi.org/10.1139/b72-026
  11. Comb. Proc. Int. Plant Prop. Soc. v.30 Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by the use of shoot top culture Lloyd,G.B.;B.H.NcCown
  12. Plant Cell Rep. v.19 Large scale growth and taxane production in cell cultures of Taxus cuspidata (Japanese yew) using a novel bioreactor Son,S.H.;M.S.Choi;Y.H.Lee;K.B.Choi;S.R.Yun;J.K.Kim;H.J.Kim;O.W.Kwon;E.W.Noh;J.H.Seon;Y.G.Park https://doi.org/10.1007/s002990050784
  13. Plant Cell Tiss. Org. Cult. v.21 Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum elaeagnifolium Cav. Nigra,H.M.;M.A.Alvarez;A.M.Giulietti https://doi.org/10.1007/BF00034492
  14. Plant Cell Rep. v.1 Production of shikonin derivertives by cell suspension cultures of Lithospermum erythrorhizon of shikonin derveritives Fujita,Y.;Y.Hara;C.Suga;T.Moromoto https://doi.org/10.1007/BF00269273
  15. Plant Cell Tiss. Org. Cult. v.18 Cell and tissue cultures of Cathararuhus roseus L. Hejiden;R.V.R.;Verpoorte;H.J.Hoopen;R.V.Heijdem;H.Tenhoopen https://doi.org/10.1007/BF00043397
  16. Phytochemistry v.16 Effects of nutritional factors on shikonin dervertive formation in Lithospermum etyrhrorhizon callus cultures Mizukami,H.;M.Konishima;M.Tabata https://doi.org/10.1016/0031-9422(77)83025-2
  17. Prog. Ind. Microbiol. v.9 The industrial potential of plant cell cultures Puchan,Z.;S.H.Martin