Biotechnology and Bioprocess Engineering:BBE

  • 제9권5호
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  • Pages.339-344
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  • 2004
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Synthesis and Characterization of Fructooligosaccharides Using Levansucrase with a High Concentration of Sucrose

  • Seo Eun-Seong (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Department of Material Chemical and Biochemical Engineering, Chonnam National University) ;
  • Lee Jin-Ha (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Engineering Research Institute, Chonnam National University) ;
  • Choi Jae-Young (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Department of Material Chemical and Biochemical Engineering, Chonnam National University) ;
  • Seo Mi-Young (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University) ;
  • Lee Hee-Sun (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Department of Physics, Chonnam National University) ;
  • Chang Seuk-Sang (Pohang Accelerator Laboratory) ;
  • Lee Hyung-Jong (Department of Molecular and Biotechnology, Chonnam National University, Biology Research Center for Industrial Accelerators, Dongshin University) ;
  • Choi Jeong-Sik (Biology Research Center for Industrial Accelerators, Dongshin University) ;
  • Kim Doman (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Biology Research Center for Industrial Accelerators, Dongshin University)
  • 발행 : 2004.10.01
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초록

A method for synthesizing branched fructo-oligosaccharides (BFOS) with a high concentration of sucrose ($1{\~}3$ M) was developed using levansucrase prepared from Leuconortoc mesenteroides B-1355C. The degree of polymerization of oligosaccharides synthesized according to the present method ranged from 2 to over 15. The synthesized BFOS were stable at a pH ranges of 2 to 4 under $120^{\circ}C$. The percentage of BFOS in the reaction digest was $95.7\%$ (excluding monosaccharides; $4.3\%$ was levan). BFOS reduced the insoluble glucan formation by Streptococcus sobrinus on the surfaces of glass vials or stainless steel wires in the presence of sucrose. They also reduced the growth and acid productions of S, sobrinus. Oligosaccharides can be used as sweeteners for foods such as beverages requiring thermo- and acid-stable properties and 3s potential inhibitors of dental caries.

키워드

참고문헌

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