Biotechnology and Bioprocess Engineering:BBE

  • 제9권5호
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  • Pages.331-338
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  • 2004
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Separation of Amino Acids by Simulated Moving Bed Using Competitive Langmuir Isotherm

  • Yang, Yun-Jeong (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Lee, Chong-Ho (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Koo, Yoon-Mo (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University)
  • 발행 : 2004.10.01
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초록

The Separation of two amino acids, phenylalanine and tryptophan, was carried out using laboratory simulated moving bed (SMB) chromatography. The SMB process consisted of four zones, with each zone having 2 columns. The triangle theory was used to obtain the operating conditions for the SMB. The mass transfer coefficients of the two amino acids were obtained from the best-fit values by comparing simulated and experimental pulse data. The competitive adsorption isotherms of the two amino acids were obtained by single and binary frontal analyses, taking into consideration the competition between the two components. A competitive Langmuir isotherm, obtained from single-component frontal chromatography, was used in the first run, and the isotherm from binary frontal chromatography in the second, with the flow rate of zone 1 modified to improve the purity. Compared to the first and second runs, the competitive Langmuir isotherm from the binary frontal chromatography Showed good agreement with the experimental results. Also, adjusting the flow rate in zone 1 increased the purity of the products. The purities of the phenylalanine in the raffinate and the tryptophan in the extract were 99.84 and $99.99\%$, respectively.

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참고문헌

  1. Broughton, D. B. (1968) Molex case history of a process. Chem. Eng. Prog. 64: 60
  2. Broughton, D. B., R. W. Neuzil, J. M. Pharis, and C. S. Brearley (1970) The Parex process for recovering paraxylene. Chem. Eng. Prog. 66: 70
  3. Hong, S. P., C. H. Lee, S. K. Kim, H. S. Yun, J. H. Lee, and K. H. Row (2004) Mobile phase compositions for ceramide 3 by normal phase high performance liquid chromatography. Biotechnol. Bioprocess Eng. 9: 47-51 https://doi.org/10.1007/BF02949321
  4. Pynnonen, B. (1998) Simulated moving bed process: Escape from the high-cost box. J. Chrornatogr. A827: 143-160
  5. Jupke, A., A. Epping, and H. Schmidt-Traub (2002) Optimal design of batch and simulated moving bed chromatographic separation processes. J. Chrornatogr. A944: 93-117
  6. Yang, Y. J., S. H. Hwang, S. M. Lee, Y. J. Kim, and Y. M. Koo (2002) Continuous cultivation of lactobacillus rhamnosus with cell recycling using an acoustic cell settler. Biotechnol. Bioprocess Eng. 7: 357-361 https://doi.org/10.1007/BF02933521
  7. Jacobson, J. M., J. H. Frenz, and C. Horvath (1987) Measurement of competitive adsorption isotherms by frontal chromatography. Ind. Eng. Chem. Res. 26: 43-50 https://doi.org/10.1021/ie00061a009
  8. Wu, D.-J., Y. Xie, Z. Ma, and N.-H. L. Wang (1998) Design of simulated moving bed chromatography for amino acid separations. Ind. Eng. Chem. Res. 37: 4023-4035 https://doi.org/10.1021/ie9801711
  9. Pais, L. S., J. M. Loureiro, and A. E. Rodrigues (2000) Chiral separation by SMB chromatography. Sep. Purif Technol. 20: 67- 77 https://doi.org/10.1016/S1383-5866(00)00063-0
  10. Kim, Y. S., C. H. Lee, Y. M. Koo, and P. C. Wankat (2003) Studies on the one-column analogue of a fourzone SMB. Proceedings of the Third Pacific Basin Conference on Adsorption Science and Technology. May 25-29, Kyongju, Korea
  11. Row, K. H., C. H. Lee, and J. H. Kang (2002) Parameter estimation of perillyl alcohol in RP-HPLC by moment analysis. Biotechnol. Bioprocess Eng. 7: 11-16
  12. Juza, M. (1999) Development of a high-performance liquid chromatographic simulated moving bed separation from an industrial perspective. J. Chrornatogr. A865: 35-49
  13. Khattabi, S., D. E. Cherrak, J. Fischer, P. Jandera, and G. Guiochon (2000) Study of the adsorption behavior of the enantiomers of 1-phenyl-1-propanol on a cellulose-based chiral stationary phase. J. Chrornatogr. A877: 95-107
  14. Huthmann, E. and M. Juza (2001) Modification of a commercial chiral stationary phase influences on enantiomer separations using simulated moving bed chromatography. J. Chrornatogr. A908: 185-200
  15. Khattabi, S., D. E. Cherrak, K. Mihlbachler, and G. Guiochon (2000) Enantioseparation of 1-phenyl-1-propanol by simulated moving bed under linear and nonlinear conditions. J. Chrornatogr. A893: 307-319
  16. Heuer, C., E. Kusters, T. Plattner, and A. SeidelMorgenstern' (1998) Designing of the simulated moving bed process based on adsorption isotherm measurements using a perturbation method. J. Chrornatogr. A827: 175-191
  17. Ma, Z. and N.-H. L. Wang (1997) Standing wave analysis of SMB chromatography: Linear systems. AIChE J. 43: 2488-2508 https://doi.org/10.1002/aic.690431012
  18. Xie, Y., Y. M. Koo, and N.-H. L. Wang (2001) Preparative chromatographic separation: Simulated moving bed and modified chromatography methods. Biotechnol. Bioprocess Eng. 6: 363-375 https://doi.org/10.1007/BF02932317
  19. Juza, M., M. Mazzotti, and M. Morbidelli (2000) Simulated moving-bed chromatography and its application to chirotechnology. Trends Biotechnol. 18: 108-118 https://doi.org/10.1016/S0167-7799(99)01419-5
  20. Mazzotti, M., G. Storti, and M. Morbidelli (1997) Optimal operation of simulated moving bed units for nonlinear chromatographic separations. J. Chromatogr. 769: 3-24 https://doi.org/10.1016/S0021-9673(97)00048-4
  21. Zhu, J., A. M. Katti, and G. Guiochon (1991) Comparison of various isotherm models for predicting competitive adsorption data. J. Chromatogr. A552: 71-89
  22. Jacobson, J. M. and J. H. Frenz (1990) Determination of competitive adsorption isotherms for modeling large-scale separations in liquid chromatography. J. Chromatogr. 499: 5-19 https://doi.org/10.1016/S0021-9673(00)96959-0
  23. Jacobson, S. C., A. Felinger, and G. Guiochon (1992) Optimizing the sample size and the reduced velocity to achieve maximum production rates of enantiomers. Biotechnol. Prog. 8: 533-539 https://doi.org/10.1021/bp00018a010