Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Screening of Bifidobacterium spp. for the Development of Infant Probiotics

유아용 생균제 개발을 위한 Bifidobacterium spp.의 선발

  • Yang, Hyun-Ju (Department of Food Science and Technology and the Research Center for Bioresource and Health, Chungbuk University) ;
  • Jang, Keum-Il (Department of Food Science and Technology and the Research Center for Bioresource and Health, Chungbuk University) ;
  • Kim, Chung-Ho (Department of Food and Nutrition, Seowon University) ;
  • Lee, Yoon-Bok (Central Research Institute, Dr. Chung's Food Company, Ltd.) ;
  • Sohn, Heon-Soo (Central Research Institute, Dr. Chung's Food Company, Ltd.) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology and the Research Center for Bioresource and Health, Chungbuk University)
  • 양현주 (충북대학교 식품공학과 및 생물건강산업개발연구센터) ;
  • 장금일 (충북대학교 식품공학과 및 생물건강산업개발연구센터) ;
  • 김정호 (서원대학교 식품영양학과) ;
  • 이윤복 ((주)정식품 중앙연구소) ;
  • 손헌수 ((주)정식품 중앙연구소) ;
  • 김광엽 (충북대학교 식품공학과 및 생물건강산업개발연구센터)
  • Published : 2004.10.31
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Abstract

Bifidobacterium spp. exhibits the highest number of counts among species of microflora in breast-feeding infant intestines and has been used as probiotics. From infant groups with different diets, 42 Bifidobacterial strains were isolated by selective plate, Gram-staining, and morphology using method of Mitsuoka, among which seven isolates were identified as Bifidobacterium spp. by F6PPK test, MIDI, and PCR. B. bifidum PBH-30, selected for development of probiotics, showed high resistance against low pH and oxgall treatment, and inhibition against pathogens such as Salmonella typhimurium and Staphylococcus aureus. B. bifidum PBH-30 could be applicable to dairy products as probiotic strains due to its excellent growth in raw milk.

본 연구에서는 유아용 생균제 개발을 위하여 1세 미만의 한국인 유아로부터 Bifidobacterium균을 분리 및 동정하여 생균제 특성이 우수한 균주를 선발하고자 하였다. 20명의 충북지역 유아 분변으로부터 선택배지를 이용한 plate법, 그람염색법을 이용하여 42종의 Bifidobacterium균을 분리하였고, F6PPK test, MIDI 및 PCR 방법을 통해 최종 7종의 Bifidobacterium균을 동정하였다. 동정된 7종의 Bifidobacterium균의 내산성 및 내담즙성 실험을 통하여 높은 효능을 나타내는 B. psedocatenulatum PBH-29와 B. bifidum PBH-30을 선발하였다. 그리고 병원성균 성장억제 실험에서 B. bifidum PBH-30이 Sal. typhimurium과 Staph. aureus에 대하여 B. psedocatenulatum PBH-29보다 효과적인 저해를 유도하였고, 10% skim milk에서도 48시간 동안 배양한 결과, B. bifidum PBH-30이 B. psedocatenulatum PBH-29보다 좀 더 높은 성장능을 나타냈다. 따라서 본 연구에서는 최종적으로 B. bifidum PBH-30을 유아용 생균제 개발을 위한 균주로 선발하였다.

Keywords

References

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