Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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An Experimental Study of Effect on ECV 304 Cells, Platelet Rich Plasma and Rats treated with L-NAME by Ondamtang extract

온담탕이 고혈압 백서와 인간유래 혈관내피세포주(ECV 304)에 미치는 영향

  • Baek Il-Sung (Department of Oriental Medicine Graduate school of Daegu Haany University) ;
  • Park Chang-Gook (Department of Oriental Medicine Graduate school of Daegu Haany University) ;
  • Lee So-Yeon (Department of Oriental Medicine Graduate school of Daegu Haany University) ;
  • Yoon Hyeon-Deok (Department of Oriental Medicine Graduate school of Daegu Haany University) ;
  • Sin Wo-Chul (Department of Oriental Medicine Graduate school of Daegu Haany University) ;
  • Park Chi-Sang (Department of Oriental Medicine Graduate school of Daegu Haany University)
  • 백일성 (대구한의대학교 한의학과 내과학) ;
  • 박창국 (대구한의대학교 한의학과 내과학) ;
  • 이소연 (대구한의대학교 한의학과 내과학) ;
  • 윤현덕 (대구한의대학교 한의학과 내과학) ;
  • 신오철 (대구한의대학교 한의학과 내과학) ;
  • 박치상 (대구한의대학교 한의학과 내과학)
  • Published : 2004.12.30
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Abstract

Nitric oxide(NO) play an important role in normal and pathophysiological cells including as a messenger molecule, neurotransmitter, microbiocidal agent, or dilator of blood vessels and artheriosclerosis, hypertension, myocardial infarction, respectively. To investigate that Ondamtang in the potential contribution of the levels of nitric oxide generated by endothelial nitric oxide synthase (eNOS) and the mechanisms of protection against L-NAME, human ECV304 cells, which normally do not express eNOS, were expressed by L-NAME. L-NAME stimulated rat or cells were found to be resistant to injury and delayed death following the Ondam-tang. Inhibition of nitric oxide synthesis abolished the protective effect against L-NAME, thrombin and collagen exposure. Interestingly, such effects have bee observed during stimulation with agents such as KCl on L-NAME mediate rats, were damaged by the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME). Cardiovascular diseases is one of the blood vessels and renin-angiotensin system dynfunction. So we studied on herbal medicine that have a relation of vessels endothelium necrosis. In Oriental Medicine, Ondam-tang has been used for disease in relation to cardiovascular system. We studied on the protection and inhibitory effects of cardiovascular diseases in L-NAME induced rat or ECV304 cell lines through the Cell morphological pattern, Tunel assay, LDH activity, heart rate, blood pressure and immunohistochemistric analysis by Ondam-tang. As the result of this study, In group, the anti-apoptosis and necrosis in the cardiovascular system have a potential capacity for prevented, protected and treating the diseases of cardiovascular system, against the necrosis of rat and ECV304 cells with eNOS and calpain expression by L-NAME is promoted.

Keywords

References

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