Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Inhibitory effect of Chelidonii Herba water extract on production of Nitric Oxide, Expression of iNOS and COX-2 in lipopolysaccharide-activated Raw 264.7 cells

백굴채(白屈菜)의 물추출물이 lipopolysaccharide로 유도된 Nitric Oxide의 생성 및 iNOS와 COX-2의 발현에 미치는 영향

  • Zhao Rong-Jie (Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University,Dept. of pharmacology, College of Medicine, Yanbian University) ;
  • Kim Young-Woo (Dept. of prescription, College of Oriental Medicine, Daegu Haany University,Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Byun Sung-Hui (Dept. of prescription, College of Oriental Medicine, Daegu Haany University,Jaehan Oriental Medical Academy, Daegu Haany University) ;
  • Kim Sang-Chan (Dept. of prescription, College of Oriental Medicine, Daegu Haany University,Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University,Jaehan Oriental Medical Academy, Daegu Haany University)
  • 조용걸 (한방생명자원연구센터,연변대학교 의과대학 약리학교실) ;
  • 김영우 (대구한의대학교 한의과대학 한의학과 방제학교실,한방생명자원연구센터) ;
  • 변성희 (대구한의대학교 한의과대학 한의학과 방제학교실,제한동의학술원) ;
  • 김상찬 (대구한의대학교 한의과대학 한의학과 방제학교실,한방생명자원연구센터,제한동의학술원)
  • Published : 2004.12.30
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Abstract

Chelidonii Herba (CHE, Baek-gul-chae in Korean), which has its original description in Gu-Hwang-Bon-Cho, a classic book of oriental Herbal book, is widely used in the treatment of stomach cancer, jaundice, gasrtic ulcer, edema and stomach pain, in Korea, Japan and China. The present study was conducted to evaluate the effect of CHE on the nitric oxide (NO) production, iNOS and COX-2 expression in lipopolysaccharide - activated Raw 264.7 cells. After the treatment of CHE, NO production was monitored by measuring the nitrite content in culture medium, cell viability was measured by MIT assay. COX-2 and iNOS were determined by lmmunoblot analysis. The production of nitric oxide was significantly inhibited by pretreatment (1h) with CHE (0.1-0.3 mg／ml) on LPS-activated Raw264.7 cells. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) protein were up-regulated by LPS, but the increased levels of iNOS and COX-2 were inhibited by pretreatment of CHE (0.1-0.3 mg／ml), respectively. Thus, the present data suggest that CHE may play an important role in adjunctive therapy in Gram-negative bacterial infections.

Keywords

References

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