야생 복숭아나무 추출물의 항산화 및 항염증 효과

Antioxidant and Antiinflammation Activities of Prunus persica Tree Extracts

  • 차배천 (상지대학교 생명자원과학대학 생명산업학과) ;
  • 이은희 (상지대학교 생명자원과학대학 생명산업학과)
  • Cha, Bae-Cheon (Dept. of Bio-Industry & Technology, Sangji Univ.) ;
  • Lee, Eun-Hee (Dept. of Bio-Industry & Technology, Sangji Univ.)
  • 발행 : 2004.09.30

초록

인류의 수명이 연장되고 노령화 사회로 발전됨에 따라 다양한 성인병이 야기되고 있고, 이들 성인병의 원인이 활성산소에 의한 것으로 밝혀짐에 따라 다양한 성인병을 예방 치료할 수 있는 안전하고 우수한 항산화 및 항염증 물질을 천연물로부터 개발하기 위한 연구의 일환으로 자생식물의 하나인 야생복숭아 가지에 대하여 그들의 MeOH 엑스를 필두로 한 분획물 엑스를 대상으로 항산화 및 항염증 효과를 조사하였다. DPPH 법을 사용하여 야생복숭아 가지의 분획물 엑스에 대한 1차 라디칼 소거작용 검색 시험을 한 결과 야생복숭아 가지 의 n-BuOH 엑스와 EtOAc 엑스가 가장 강한 항산화 활성을 나타내었다. 1차 실험에서 얻어진 결과를 토대로 야생복숭아에 대하여 보다 상세한 항산화 효과를 검토하기 위하여 Ferric-Thiocyanate 법을 이용한 지질 과산화 억제작용 시험한 결과 야생복숭아 가지 의 EtOAc 엑스가 천연 항산화제인 tocopherol 및 합성 항산화제인 BHA 보다 우수한 탁월한 항산화 효과를 나타내었고, Rancimat법에 의한 지질 산패 억제 실험을 실시한 결과 또한 야생복숭아 가지의 EtOAc 엑스가 tocopherol보다 우수하고 BHA보다는 약간 우수한 강력한 지질 산패 억제 효과를 보였다. Hyaluronidase 저해에 의한 항염증 효과 시험에서는 항산화 실험에서 전반적으로 우수한 항산화 효과를 보인 야생복숭아 가지의 EtOAc 엑스가 ketoprofen보다는 약하나 ibuprofen과 indomethacin보다는 우수한 항염증 효과를 나타내었다. 이상의 실험 결과는 야생복숭아의 항산화 및 항염증 효과의 활성 주성분 분획은 EtOAc 엑스임을 시사하였다.

Reactive oxygen species (ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. In this study, we have conducted to investigate the antioxidant and hyaluronidase inhibitory activities of Prunus persica Batsch var. davidiana Maximowicz in order to screen the bioactive substances which can be developed as possible anti-inflammatory agents. Among the extracts of Prunus persica Batsch var. davidiana Maximowicz, EtOAc extract exhibited the strongest effect on antioxidant experiment such as DPPH, Ferric- Thiocyanate and Rancimat. Also, EtOAc extract showed a potent hyaluronidase inhibitory activity.

키워드

참고문헌

  1. Branen AL (1975) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. JAOCS. 52:59-63 https://doi.org/10.1007/BF02901825
  2. Chen CW, Ho CT (1995) Antioxidant properties of polyphenols extracted from green and black teas. J. Food Lipids 2:35-46 https://doi.org/10.1111/j.1745-4522.1995.tb00028.x
  3. Choi JS, Lee JH, Park HJ, Kim HG, Young HS, Mun SI (1993) Screening for antioxidant activity of plants and marine algae and its active principles from Prunus davidiana. Korean J. Pharmacogn. 24:299-303
  4. Choi JS, Yokozawa T, Oura H (1991) Antihyperlipidemic effect of flavonoids from Prunus davidiana. J. Nat. Prod. 54:218-224 https://doi.org/10.1021/np50073a022
  5. Chung IM, Kim KH, Ahn JK (1998) Screening of korean medicinal and food plants with antioxidant activity. Korean J. Medicinal Crop Sci. 6:311-322
  6. Coleman MD, Fernandes S, Khanderia LA (2003) A preliminary evaluation of a novel method to monitor a triple antioxidant combination (vitamins E, C and $\alpha$lipoic acid) in diabetic volunteers using in vitro methaemaglobin formation. Environ. Toxicol. Pharmacol. 14:69-75 https://doi.org/10.1016/S1382-6689(03)00027-9
  7. Corl MM (1974) Antioxidant activity of tocopherol and ascorbyl palmitate and their mode of action. JAOCS 51:321-325 https://doi.org/10.1007/BF02633006
  8. Fukuzawa K, Takaishi Y (1990) Antioxidants. J. Act. Oxyg. Free Rad. 1:55-70
  9. Ghosh P (1994) The role of hyaluronic acid(hyaluronan) in health and disease: interactions with cells, cartilage and components of synoval fluids, Clin. Exp. Rheumatol. 12:82-85
  10. Halliwell B (1991) Drug antioxidant effects. Drugs 42:569-605 https://doi.org/10.2165/00003495-199142040-00003
  11. Hanninen O, Kaartinen K, Rauma AL, Nenonen M, Torronen R, Hakkinen S, Adlercreutz H, Laakso J (2000) Antioxidants in vegan Diet and rheumatic disorders. Toxicology 155:45-53 https://doi.org/10.1016/S0300-483X(00)00276-6
  12. Inatani R, Nakatani N, Fuwa H (1963) Antioxidative effect of the constituents of rosemary(Rosmarinus officinalis L.) and their derivatives. Agric. Biol. Chem. 47:521-528
  13. Jeong SJ, Ko YS, Ahn NH, Kim YC (1998) Hyaluronidase inhibitor from Uncariae famulus et Uncus. Korean J. Phamacogn. 29:169-172
  14. Kim YS, Noh YK, Lee GI, Kim YK, Lee KS, Min KR (1995) Inhibitory effects of herbal medicines on hyaluronidase activity. Korean J. Pharmacogn. 26:265-272
  15. Lee NH, Lee SJ, Jung DS, Bu HJ, Yang HC, Riu KZ (2001) Screening of the tyrosinase inhibition and hyaluronidase inhibition activities, and radical scavenging effects using plants in Cheju, Korean J. Pharmacogn. 32:175-180
  16. Lee SE, Hwang HJ, Ha BS, Jeong HS, Kim JH (2003) Screening of medicinal plant extracts for antioxidant activity. Life Sci. 73:167-179 https://doi.org/10.1016/S0024-3205(03)00259-5
  17. Lim DK., Choi U, Shin DH (1996) Antioxidative actibity of some solvent extract Caesalpinia sappan L. Korean J. Food Sci. Technol. 28:77-82
  18. Marquina MA, Corao GM, Araujo L, Buitrago D, Sosa M (2002) Hyaluronidase inhibitory activity from the polyphenols in the fruit of blackberry (Rubus fruticosus B.). Fitoterapia 73:727-729 https://doi.org/10.1016/S0367-326X(02)00222-8
  19. Meyer K (1947) The biological significance of hyaluronic acid and hyaluronidase. Physiol. Rev. 27:335-359 https://doi.org/10.1152/physrev.1947.27.3.335
  20. Miliauskas G, Venskutonis PR, Van-Beek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 85:231-237 https://doi.org/10.1016/j.foodchem.2003.05.007
  21. Monsen ER (2000) Dietary reference intakes for the antioxidant nutrients: vitamin C, vitamin E, selenium, and carotenoids. J. Am. Diet. Assoc. 100:637-640 https://doi.org/10.1016/S0002-8223(00)00189-9
  22. Olas B, Wachowicz B (2002) Resveratrol and vitamin C as antioxidants in blood platelets. Thromb. Res. 106:143-148 https://doi.org/10.1016/S0049-3848(02)00101-9
  23. Papa S, Skulachev VP (1997) Reactive oxygen species, mitochondria, apoptosis and aging. Mol. Cell Biochem. 174:305-319 https://doi.org/10.1023/A:1006873518427
  24. Rehman Z, Habib F, Shah WH (2004) Utilization of potato peels extract as a natural antioxidant in soy bean oil. Food Chemistry 85:215-220 https://doi.org/10.1016/j.foodchem.2003.06.015
  25. Uchiyama M, Suzuki Y, Fukuzawa K (1968) Biochemical studies of physiological function of tocopheronolactone. 1. Yakugaku Zasshi 88:678-683 https://doi.org/10.1248/yakushi1947.88.6_678
  26. Uematsu C, Sasakuma T, Ogihara Y (1991) Phylogenetic relationships in the stone fruit group of Prunus as revealed by restriction fragment analysis of chloroplast DNA. Japan J. Genet. 66:59-69 https://doi.org/10.1266/jjg.66.59
  27. Yoshikawa M, Harada E, Miki A, Tsukamoto K, Liang SQ, Yamahara J, Murakami N (1994) Antioxidant constituents from the fruit hulls of Mangosteen (Garcinia mangodtana L.) originating in Vetnam. Yakugaku Zasshi 114:129-133 https://doi.org/10.1248/yakushi1947.114.2_129
  28. 생약학연구회 (1994) 현대생약학. 학창사. 서울. p. 322-323
  29. 木村善行, 興田拓道 (1998) 抗酸化劑としての和漢藥. 日本臨床 46:2286-2292
  30. 皆川信子 (1993) 活性酸素が關與すゐ代表的疾患. フアルマシア 29:1029-1030