생물정신의학 (Korean Journal of Biological Psychiatry)
- 제10권1호
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- Pages.62-69
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- 2003
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- 1225-8709(pISSN)
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- 2005-7571(eISSN)
족부전기충격이나 족부전기충격과 연합-학습된 조건자극에 재노출시 흰쥐뇌내 Corticotropin-Releasing Factor(CRF)와 Neuropeptide Y(NPY)의 변동에 관한 연구
Changes of Corticotropin-Releasing Factor(CRF) and Neuropeptide Y(NPY) of Rats in Response to Footshock or Reexposure to Conditions Previously Paired with Footshock
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신경호
(고려대학교 의과대학 약리학교실) ;
- 김성진 (고려대학교 의과대학 약리학교실) ;
- 이금주 (고려대학교 의과대학 약리학교실) ;
- 신승건 (고려대학교 의과대학 약리학교실) ;
- 신유찬 (고려대학교 의과대학 약리학교실) ;
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이민수
(고려대학교 의과대학 신경정신과학교실)
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Shin, Kyung-Ho
(Department of Pharmacology, Korea University College of Medicine) ;
- Kim, Sung Jin (Department of Pharmacology, Korea University College of Medicine) ;
- Lee, Kuem Ju (Department of Pharmacology, Korea University College of Medicine) ;
- Shin, Seung Gun (Department of Pharmacology, Korea University College of Medicine) ;
- Shin, You Chan (Department of Pharmacology, Korea University College of Medicine) ;
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Lee, Min-Soo
(Department of Psychiatry, Korea University College of Medicine)
- 발행 : 2003.06.30
초록
Corticotropin-releasing factor(CRF) and neuropeptide Y(NPY) are known to play important roles in mediating stress responses and stress-related behavior. To elucidate the role of neuropeptides in response to the condition that had paired with traumatic event, we observed the changes of CRF and NPY by immunohistochemistry using a conditioned footshock paradigm. Male Sprague-Dawley rats were placed in a shuttle box and exposed to 20 pairings of a tone(< 70dB, 5sec) followed by a footshock(FS, 0.8mA, 1sec) over 60min. A second group was exposed to the tone-footshock pairings, returned to the homecage for 2days, and then reexposed to the test chamber and 20tones alone for 60min, prior to sacrifice. Control groups were : a) sacrificed without exposure to FS ; b) exposed to the tone-footshock pairings and then sacrificed two days later ; or c) exposed to the chamber and tones alone, returned to the homecage for 2days and then reexposed to the chamber and 20tones over 60min prior to sacrifice. CRF was increased in animals exposed to FS or the aversive condition(context and tone) that had paired to FS in bed nucleus of the stria terminalis (BNST) compared to the control. NPY was increased by FS in amygdala and PVN, but the condition previously associated with FS results in slight increase only in amygdala area. These results suggest that the BNST appears to be the mostly involved neural circuit in response to explicit cues previously paired with footshock. Moreover, this study raise the possibility that increased CRF peptide in the BNST in response to re-exposure to the aversive condition may underlie, in part, the experience of conditioned fear-related anxiety behavior.