IMMUNE NETWORK
- Volume 3 Issue 2
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- Pages.136-144
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- 2003
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
Studies on the Cellular Immune Response in Animal Model of Arthritis after the Induction of Oral Tolerance
콜라겐으로 경구 관용을 유도한 관절염 동물 모델의 세포 특이적 면역 반응 조사
- Min, So-Youn (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Hwang, Sue-Yun (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Lee, Jae-sun (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Kim, Ju-Young (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Lee, Kang-Eun (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Kim, Kyung-Wun (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Kim, Young-Hun (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Do, Ju-Ho (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea) ;
- Kim, Ho-Youn (Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea)
- 민소연 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 황수연 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 이재선 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 김주영 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 이강은 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 김경운 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 김영훈 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 도주호 (가톨릭대학교 의과학연구원 류마티스연구센터) ;
- 김호연 (가톨릭대학교 의과학연구원 류마티스연구센터)
- Published : 2003.06.30
Abstract
Oral administration of antigen has long been considered as a promising alternative for the treatment of chronic autoimmune diseases including rheumatoid arthritis (RA), and oral application of type II collagen (CII) has been proven to improve pathogenic symptoms in RA patients without problematic side effects. To further current understandings about the immune suppression mechanisms mediated by orally administered antigens, we examined the changes in IgG subtypes, T-cell proliferative response, and proportion of interleukin (IL)-10 producing Th subsets in a time course study of collagen induced arthritis (CIA) animal models. We found that joint inflammation in CIA mouse peaked at 5 weeks after first immunization with CII, which was significantly subdued in mice pre-treated by repeated oral administration of CII. Orally tolerized mice also showed increase in their serum level of IgG1, while the level of IgG2a was decreased. T-cell proliferation upon CII stimulation was also suppressed in lymph nodes of mice given oral administration of CII compared to non-tolerized controls. When cultured in vitro in the presence of CII, T-cells isolated from orally tolerized mice presented higher proportion of
Keywords
- Oral tolerance;
- rheumatoid arthritis;
- type II collagen;
- interleukin-10 (IL-10);
- interferon-gamma ($IFN-{\gamma}$)