Journal of dental hygiene science (치위생과학회지)
- Volume 3 Issue 1
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- Pages.11-14
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- 2003
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- 1598-4478(pISSN)
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- 2233-7679(eISSN)
Purification of Materials Produced by Amylocolatosis sp. and Anticancer Effect in Oral Cancer Model
Amylocolatosis sp.가 생산하는 항암물질의 정제 및 구강암 모델에 미치는 항암 효과
- Kim, Jung (Department of Dental Hygiene, Suwon Women's College) ;
- Park, Young-Min (Department of Dental Hygiene, Yeojoo Institute of Technology(YIT)) ;
- Lim, Do-Seon (Department of Dental Hygiene, Seoul Health College)
- Received : 2003.05.21
- Accepted : 2003.06.16
- Published : 2003.06.30
Abstract
A methylotrophic Actinomycetes strain, which produce the anti-oral cancer activity compound, was isolated from soil and estimated as Amylocolatosis sp. based on taxonomic studies. A methanol didn't have influence on the production of the anticancer compounds. These compound were isolated by ethylacetate extract, silica gel column chromatography, sephadex LH-20 column and reverse phase HPLC. The compounds were very stable under heat (
각종 토양시료로부터 M배지와 AMP배지에서 생육하는 500여종의 방선균을 분리하였으며, 그 중 전배양배지(GMY 배지)에서 가장 뛰어난 항암활성능을 소유한 균주를 불리하고 배양하여 세포외 항암성물질을 분리하여 이 물질을 완전정제하고 MTT 정량분석을 실시하여 암세포에 대한 세포독성검사를 실시하였다. 1. 정제는 배양한 균체를 완전히 제거하고 동량의 ethylacetate를 처리하여 배양액중의 항암성분을 추출하고 무수 magnesium sulfate로 건조 후 농축, ethanol로 용해, 10배량의 water을 첨가하여