Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

High Frequency Induction of Multiple Shoots from Nodal Explants of Vitex negundo L. Using Sodium Sulphate

  • Chandramu C. (Department of Genetics, Osmania University) ;
  • Rao D. Manohar (Department of Genetics, Osmania University) ;
  • Reddy V. Dashavantha (Center for Plant Molecular Biology, Department of Genetics, Osmania University)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of sodium sulphate on shoot induction and multiple shoot formation from nodal explants of Vitex negundo L. was tested on Murashige and Skoog's (MS) medium fortified with different auxins, cytokinins and sucrose. Highest percentage $(97.78\%)$ of explants for shoot induction and multiple shoot (20.68/explant) production were observed in the combination treatment of $N^6-Benzyl$ adenine (BA) $(17.80\;{\mu}M/L)$, ${\alpha}-Naphthalene$ acetic acid (NAA) $(2.15\;{\mu}M/L)$ and $5\%$ sucrose supplemented with 100 mg/L sodium sulphate. In vitro raised shoots were rooted on the half-strength MS medium fortified with different concentrations of NAA, Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) alone and in combinations. Among the treatments, $4.90\;{\mu}M/L$ of IBA was found most effective $(95.56\%)$ in inducing roots. The rooted plantlets were shifted to glasshouse for acclimatization and later transferred to the field with cent percent survival. Furthermore, in vitro flowering was observed in the shoots cultured on MS medium supplemented with BA $(8.90\;{mu}M/L)$ and NAA $(1.61\;{\mu}M/L)$.

Keywords

References

  1. The Useful Plants of India Ambasta SP
  2. Indian J Expt Biol v.34 Screening of Indian plants for biological activity: Part XV Aswal BS;Goel AK;Kulshrestha DK;Mehrotro BN;Patnaik G K
  3. Plant Cell Rep v.18 In vitro multiplication of cashew (Anachardium occidentale L.) using shoot node explants of glasshouse- raised plants Boggetti B;Jasik J;Mantell S https://doi.org/10.1007/s002990050603
  4. Wealth of India, A dictionary of Indian raw materials and Industrial Products v.Ⅹ(Sp - W) Chadha
  5. Plant Physiol v.84 Characterization of Growth, water relations and proline accumulation in Sodium sulphate tolerant callus of Brassica napus L. cv. Wester (candla) Chandler SF;Thorpe TA https://doi.org/10.1104/pp.84.1.106
  6. Tebin J Plant Physiol v.126 Effect of sodium sulphate on tissue culture of Brassica napus cv. Westar and Brassica campestris cv. Chandler SF;Mandal BB;Thorpe TA https://doi.org/10.1016/S0176-1617(86)80223-1
  7. Phytotherapy Research v.16 Isolation, characterization and Biological activity of betulinic acid and ursolic acid from Vitex negundo L. Chandramu C;Rao DM;Krupadanam GLD;Reddy VD
  8. Indian J Chem v.30 Chemical investigation and anti-inflammatory activity of Vitex negundo seeds: Part-I Chawla AS;Sharma AK;Hamda SS;Dhark L
  9. Planta Med v.68 Angiogenic Activity of β-Sitosterol in the Ischaemia /Reperfusion-Damaged Brain of Mongolian Gerbil Choi S;Kim KW;Choi JS;Han ST;Park YI;Lee SK;Kim JS;Chung MH https://doi.org/10.1055/s-2002-26750
  10. J Natl Prod v.57 Anti-AIDES agents-11: Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoides Fujioka T;Kashiwada Y;Kilkuskie RE;Cosentino CM;Ballas LM;Jiang JB;Janzen WP;Chen IS;Lee KH https://doi.org/10.1021/np50104a008
  11. J Sci Ind Res v.6 Investigations on plant antibiotics: Part- II-A search for antibiotic substances in some Indian medicinal plants George M;Venkataraman PR;Pundalai KM
  12. Indian J Med Res [A] v.95 Mosquito repellant activity of oils from Vitex negundo L. leaves. Hebbalkar DS;Hebbalkar GD;Sharma RN;Joshi VS;Bhat VS
  13. Calif Agric Sta Circ No 347 The water culture method for growing plants without soil Hoaglands DR;Arnon PS
  14. Dictionary of Indian Medicinal Plants Husain A;Virmani CP;Popli SP;Misra LN;Gupta MM;Srivastava GN;Abraham Z;Singh AK
  15. Indian J Pharm Sci v.56 Hepatoprotective activity of “JIGRINE” (An unani polypharmaceutical herbal formulation) on liver damage and lipid peroxidation caused by alcohol-carbon tetrachloride and paracetamol in rats Kapur V;Pillai KK;Hussain SZ;Balani DK
  16. Plant Cell Rep v.16 Highfrequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. by thidiazuron Kim MK;Sommer HE;Bongarten BC;Merkle SA
  17. Plant Tissue Culture and Biotechnology Emerging Trends Sodium, Potassium, Sugar alcohol and Proline mediated somatic embryogenesis and plant regeneration in recalcitrant rice callus Kishor PBK;Sangam S;Naidu KR;Kishore P B K (ed.)
  18. Plant Cell Rep v.16 An efficient in vitro method for mass propagation of a woody ornamental plant Ixora coccinea L. Lakshmanan P;Lee CL;Goh CJ
  19. Plant Cell Tiss Org Cult v.10 Establishment and multiplication of colchicine- autotetraploids of Rauvolfia serpentina L. Mathur A;Mathur AK;Kukreja AK;Ahuja;Tyagi BR https://doi.org/10.1007/BF00035910
  20. Plant Cell Tiss Org Cult v.28 Tissue culture plant propagation of Gomphrena officinalis, a branzillian medicinal plant Mercier H;Vieira CCJ;Riberiro RCLF https://doi.org/10.1007/BF00036120
  21. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige T;Skoog F https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  22. Chem Pharm Bull v.45 Enhanced cytotoxicity of some triterpenes towards leukemia L 1210 cells cultured in low pH media Noda Y;Kaiya T;Kohda K;Kawazoe Y https://doi.org/10.1248/cpb.45.1665
  23. Plant Cell Rep v.15 In vitro propagation of medicinal herbs Ocimum americanum L. Syn. O. canum Sims (hoary basil) & O. sanctum L (holy basil) Pattnaik SK;Chand PK https://doi.org/10.1007/BF00233154
  24. Plant Cell Rep v.16 Rapid clonal propagation of three mulberries, Morus cathayana Hemsl., M. lhou Koiz., and M. serrata Roxb., through in in vitro culture of apical shoot bud and nodal explants from mature trees Pattnaik SK;Chand PK
  25. Indian J Pharm Sci no.Jan;Feb Antimicrobial activity of same Indian medicinal plants Rusia K;Srivastava SK
  26. Plant Cell Rep v.18 Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high frequency axillary shoot proliferation Sahoo Y;Chand PK https://doi.org/10.1007/s002990050576
  27. Plant Cell Tiss Org Cult v.26 Micropropagation of Withania somnifera from germinating seeds and shoot tips Sen J;Sharma AK https://doi.org/10.1007/BF00036108
  28. Plant Sci v.88 Proline, thioproline and potassium induced stimulation of somatic embryogenesis in alfalfa (Medicago sativa L) Shetty, Mc;Kersic BD https://doi.org/10.1016/0168-9452(93)90090-M
  29. Plant Sci v.84 Stimulation of Benzyladenine induced in vitro shoot organogenesis in Cucumis melo L. by proline, salicylic acid and aspirin Shetty K;Shetty GA;Nakazaki Y;Yoshioka K;Asano Y;Oosawa K https://doi.org/10.1016/0168-9452(92)90134-8
  30. Statistical Methods Snedecor GW;Cochran WG
  31. Plant Cell Rep v.11 In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb) Swamy BVR;Himabindi K;Sita LG
  32. Indian J Pharm Sci Anti-arthritic activity of Vitex negundo L. Tamhankar CP;Saraf MN
  33. J Plant Biotech v.2 Masspropagation of Vitex negundo L; In vitro Thiruvengadam M;Jayabalan N
  34. Plant Cell Biotech Mol Biol v.2 no.1;2 In vitro flowering of Vitex negundo L, a medicinal plant Thiruvengadam M;Jayabalan N
  35. Plant Cell Biotech Mol Biol v.2 no.1;2 In vitro micropropagation of Dictyospermum ovalifolium Wight, a rare and endemic medicinal plant in Western Ghats India Thoyajaksha;Rai VR
  36. Plant Cell Rep v.13 Production of cucumber fruits from the culture of “marketmore”-76 plantlets Tisseral B;Galletta PD
  37. Plant Cell Tiss Org Cult v.28 Micropropagation of Kaempferia galanga L.-a medicinal plant Vincent KA;Mathew KM;Hariharan M https://doi.org/10.1007/BF00055522
  38. J Natl Prod v.59 Anti-HIV Triterpene acids from Geum japonicum Xu HX;Zeng FQ;Wan M;Sim KY https://doi.org/10.1021/np960165e