Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Cultural Characteristics and Phylogenic Relationships of Collected Strains of Pholiota species

  • Published : 2003.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Cultural characteristics and phylogenic relationships were investigated and classified among collected strains in Pholiota spp. which contain P. adiposa, P. squarrosa, P. nameko etc. They were tested on the four different media(PDA, MCM, YM, MEA) and sawdust(Alder, Oak, Pine, Popular) substrates. There was a little variation according to the media and sawdust substrates, although PDA and popular sawdust substrate seemed to be better. Most strains showed white colonies, but some strains were brown. Mycelial growth length differed according to the strains. To classify species, the internal transcribed spacer regions(ITS) of the ribosomal DNA(rDNA) repeats from Pholiota spp. were amplified using polymerase chain reaction(PCR) and then sequenced. According to the analysis of ITS sequences, they were classified into five clusters. Their spacer regions were $644{\sim}700$ nucleotides in length. The reciprocal homologies of each ITS region among these strains were ranged from $49.6{\sim}99.9%$. The phylogenic analysis might give a criterion to classify species in the collected strains.

Keywords

References

  1. Bae, S. C., Seong, K. Y., Lee, S. W., Go, S. J., Eun, M. Y. and Rhee. 1996. Phylogenetic relationships among Pleurotus species inferred from sequence data of PCR amplified ITS region in ribosomal DNA. Kor. J. Mycol. 24: 155-165
  2. Bannister, J. V., Bannister, W. and Rotilio, H. G. 1987. Aspects of the structure, function and applications of superoxide dismutase, CRC Crit. Rev. Biochem. 161: 559-566
  3. Choi, I. Y., You, Y. J., Choi, J. S. and Lee, W. H. 2000. Genetic relationships of internal transcribed spacer (ITS) regions on entomopathogenic fungi by RFLP. Kor. J. Mycol. 28(2): 112-117
  4. Chung, J. W., Kim, G. Y., Ha, M. G. Lee, T. H. and Lee, J. D. 1999. Phylogenic analysis of the genus Phellinus by comparing the sequences of internal transcribed spacers and 5.8S ribosomal DNA. Kor. J. Mycol. 27(2): 124-131
  5. Chung, K. S. 1982. Studies on constituents and culture of the higher fungi of Korea (II). Kor. J. Mycol. 10(1): 33-39
  6. Cutler, R. G. 1984. Antioxidants, aging, and longevity. 6: 371-423. In W. A. Pryor (ed.) Free radicals in biology. Academic press, Orlando, FL
  7. Graham, G. C. 1994. A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotech. 16(1): 49-50
  8. Jacobsson, S. 1989. Studies on Pholiota in culture. Mycotaxon. 36: 95-145
  9. Lee, G. Y. 1988. Colored Korean mushrooms. Academy publishing Co., Ltd. Seoul. Korea
  10. Mizuno, T. 1994. Food function and medicinal effect of mushroom fungi. Department of applied biological chemistry, Shizuoka university
  11. Park, D. S., Go, S. J. and Ryu, J. C. 1999. Phylogenetic relationships of coprinoid taxa and an agaric-like gastroid taxon based on the sequences of internal transcribed spacer (ITS) regions. Kor.J. Mycol. 27(6): 406-411
  12. Park, D. S., Go, S. J., Kim, Y. S., Seok, S. J., Ryu, J. C. and Sung, J. M. 1999. Phylogenetic relationships of genera Coprinus and Psathyrella on the basis of ITS region sequences. Kor. J. Mycol. 27(4): 274-279
  13. Park, D. S., Go, S. J., Ryu, J. C. and Sung, J. M. 1999. Phylogenetic study of Ganoderma spp. based on the DNA sequences in ITS region. Kor. J. Mycol. 27(1): 39-43
  14. Sung, J. M., Lee, J. K. and Park, D. S. 1998. The fruit-body formation and properties of Pholiota sp. Kor. J. Mycol. 26: 194-199
  15. Taylor, J. W., Jacobson, D. J., Kroken, S., Kasuga, T., Geiser, D. M., Hibbett, D. S. and Fisher, M. C. 2000. Phylogenetic species recognition and species concepts in Fungi. Fungal Genetics and Biology. 31: 21-32 https://doi.org/10.1006/fgbi.2000.1228

Cited by

  1. Pholiota chocenensis—a new European species of section Spumosae (Basidiomycota, Strophariaceae) vol.13, pp.2, 2014, https://doi.org/10.1007/s11557-013-0926-2