Mycobiology

The Korean Society of Mycology (한국균학회)
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Mycoflora and Mycotoxins of Cereal Grains in Delta, Egypt

  • Soliman, Hoda M. (Botany Department, Faculty of Science, Mansoura University)
  • Published : 2003.12.31
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Abstract

Five cereal grains(wheat, barley, rice, maize and sorghum) were collected from three Egyptian provinces known to be grain producers(Daqahlia, Gharbia and Kafer el-Sheikh). Two species of Alternaria(A. raphani and A. tenusinae); two species of Aspergillus(A. flavus and A. niger); one species of Cunninghamella(C. elegans); one Dreschslera species(D. myakt); three Fusarium species(F. graminearum, F. moniliform and F. solani); one Rhizopus species(R. stolonifer) and two species of Penicillium(P. digitatum and P. notatum) were isolated from the grains. The densities of these fungi and their frequencies of occurrence have been investigated. All the fungal isolates were tested for the production of toxic metabolites in culture media and the percentages of toxigenic isolates were calculated. The biological assay of the toxigenic fungal isolates showed significant variations in toxigenic activity. Thin layer chromatography revealed that the most active isolate produces moniliformin in culture media. The effect of culture conditions on the production of moniliformin was studied.

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References

  1. Abdel-Hafez, S. I. 1984. Composition of the fungal flora of four cereal grains in Saudi arabia. Mycopathologia 85(1-2): 53-57 https://doi.org/10.1007/BF00436702
  2. Abdel-Hafez, S. I., Moubasher, A. H., Shoreit, A. A. and Ismail, M. A. 1990. Fungal flora associated with combine harvester wheat and sorghum dusts from Egypt. J. Basic Microbiol. 30(7): 467-479 https://doi.org/10.1002/jobm.3620300702
  3. Abramson, D., Hulasare, R., White, N. D. G., Jayas, D. S. and Marquardt, R. R. 1999. Mycotoxin formation in hulless barley during granary storage at 15 and 90 moisture content. Journal of Stored Products Research 35: 297-305 https://doi.org/10.1016/S0022-474X(99)00013-2
  4. Ackermann, A. 1998. Mycoflora of south african barley and malt. J. Am. Soc. Brew. Chem. 56(4): 169-176
  5. Adisa, A. 1994. Mycoflora of post-harvest maize and wheat grains and the implication of their contamination by molds. Nahrung 38(3): 318-326 https://doi.org/10.1002/food.19940380312
  6. Almeida, A. P., Correa, B., Mallozzi, M. A. B., Sawazaki, E. and Soares, L. M. V. 2000. Mycoflora and aflatoxin/fumonisin production by fungal isolates from freshly harvested corn hybrids. Brazilian Journal of Microbiology 31: 321-326
  7. Almeida, A. P., Fonseca, H., Fancelli, A. L., Direito, G. M., Ortega, E. M. and Correa, B. 2002. Mycoflora and fumonisin contamination in Brazilian corn from sowing to harvest. J. Agric. Food Chem. 50(13): 3877-3882 https://doi.org/10.1021/jf011531p
  8. Aziz, N. H. 1987. Etiology of toxin-producing fungi from the class of deutromycetes occurring in various feed products. Ph.D. thesis. Agriculture University, Cracow, Poland
  9. Aziz, N. H., Youssef, Y. A., El-fouly, M. Z. and Moussa, L. A. 1998. Contamination of some common medicinal plant samples and spices by fungi and their mycotoxins. Bot. Bull. Acad. Sci. 39: 279-285
  10. Christensen, C. H. and Kaufmann, H. H. 1969. Grain storage: The role of fungi in quality loss The Univ. Minnesota Press. Minnesota, USA, 159 pp
  11. Christensen, C. M. 1987. Field and storage fungi In: Food and Beverage Mycology $2^{nd}\$ edition L.R. Beuchat, ed. Van Nostrand Reinhold, New York: pp. 211-232
  12. Clevsrton, G. and Ljunggren, H. 1995. Aflatoxin formation and the dual phenomenon. Mycopathologia 92: 129-139 https://doi.org/10.1007/BF00437624
  13. Czerwiecki, L., Czajowska, D. and Witkowska-Gwiazdowska, A. 2002. On ochratoxin A and fungal flora in Polish cereals from conventional and ecological farms. Part 2: Occurrence of ochratoxin A and fungi in cereals in 1989. Food additives and contaminations 19(11): 1051-1057
  14. Davis, N. D. and Diener, U. L. 1987. Mycotoxins. In: Food and Beverage Mycology. 2nd edition. L. R. Beuchat, ed. Van Nostrand Reinhold, New York: pp. 517-570
  15. Domsch, K. H., Gams, W and Anderson, T. H. 1981. Compendium of Soil Fungi vol. 1 and 2, Academic Press, London
  16. El-Kady, I. A., Abdel-Hafez, S. L. and El-maraghy, S. S. 1982. . Contribution to the fungal flora of cereal grains in Egypt. Mycopathologia 77(2): 103-109 https://doi.org/10.1007/BF00437392
  17. El-Shayeb, N. M., Mabrouk, S. S. and Abd-el fattah, A. M. 1992. Production of ochratoxins by some Egyptian Aspergillus strains. Zentralbl. Mikrobiol. 147(1-2): 86-91
  18. Flannigan, B. 1977. Enumeration of fungi and assay for ability of degrade structural and storage components of grain. In: Biodeterioration investigation techniques. A. H. Walter, ed. Applied Science, London: pp. 185-199
  19. Fotso, J., Leslie, J. F. and Smith, J. S. 2002. Production of beauvericin, moniliformin, fusaproliferin, and fumonisins b(1), b(2) and b(3) by fifteen ex-type strains of Fusarium species. Appl. Environ. Microbiol. 68(10): 5195-5197 https://doi.org/10.1128/AEM.68.10.5195-5197.2002
  20. Fraber, J. M., Sanders, G. W, Lawrence, G. A. and Scott, P. M. 1988. Production of moniliformin by Canadian isolates of Fusarium. Mycopathologia 101: 187-190 https://doi.org/10.1007/BF00437038
  21. Hernandez, E., Vila, R. and Garcia de la Cuadra, J. 1968. Microflora del arroz elaborado. 1: Flora de molinos industrials. Agroquimica Y Technologia de los Alimentos, 8(2): 240-248
  22. Javis, B. and Williams, A. P. 1987. Methods for detecting fungi in foods and beverages. In: Food and Beverage Mycology. $2^{nd}$ edition L. R. Beuchat, ed. Van Nostrad Reinhold, New York: pp. 599-636. 1987
  23. Kacaniova, M. and Tancinova, D. 2001. Natural occurrence of fungi in feeding wheat in the agricultural farm facilities. Acta Fytotechnica et Zootechnica 4: 174-175
  24. Li, F. Q., Toyazaki, N. and Yoshizawa, T. 2001. Production of Alternaria mycotoxins by Alternaria alternata isolated from weather damaged wheat. Journal of Food Protection 64: 567-571
  25. Lillehoj, E. B. and Elling, F. 1983. Environmental conditions that facilitate ochratoxin contamination of agricultural commodities. Acta. Agric. Scand. 33: 113-
  26. Marasas, W. F. O., Wehner, F. C., van Rensburg, S. J. and van Schalkwyk, D. J. 1981. Mycoflora of corn produced in human esophageal cancer area in Tanskei, Southern Africa. Phytopathology 71: 792-796 https://doi.org/10.1094/Phyto-71-792
  27. Marin, S., Sanchis, V., Saenz, R., Ramos, A. J., Vinas, I. and Magan, N. 1998. Ecological determinants for germination and growth of some Aspergillus and Penicillium spp. From maize grain. J. Appl. Microbiol. 84: 25-36
  28. Mediavilla, M. A., Angulo, R. J., Rodero, F. J. M., Dominguez, V. E., Galan, S. C. and Infante, G. F. 1996. Fungal contamination of potential medical interest in Spanish grain stores. J. lnvestig. Allergol. Clin. Immunol. 6(3): 196-201
  29. Megalla, S. E.m Abdou, R. F. and Bagy, M. M. 1985. Fungal flora of Egyptian baladi bread with special reference to the mutagenic effects of their toxic metabolites. Mycopathologia 89(1): 35-41 https://doi.org/10.1007/BF00437130
  30. Navi, S. S., Bandyopadhyay, R., Hall, A. J. and Bramel-Cox, P. J. 1999. A pictorial guide for the identification of mold fungi on sorghum grain. Information Bulletin no. 59. Patancheru 502 324, Andhra Pradesh, India: International crop Research Institute for the Semi-arid Tropics. 118 pp
  31. Nelson, P. E., Toussoun, T. A. and marasas, W. F. O. 1983. Fusarium species: An illustrated manual for Identification. The Penn. St. Univ. Press, Univ. Park, Pennsylvania, 203 pp
  32. Petters, H. I., Flamingan, B. and Austin, B. 1988. Quantitative Qualitative studies of the microflora of barley malt production. J. App. Bacteriol. 65: 279-297
  33. Pitt, J. I. 1979. The genus Penicillium and its teleomorphic states: Eupenicillium and Talaromyces. Academic Press Inc. London, 634 pp
  34. Pitt, J. I. 1985. Alaboratory guide to common Penicillium species. Commonwealth Mycological Institute, Kew, Surrey, England, 184 pp
  35. Raper, K. B. and Fennel, D. I. 1977. The Genus Aspergillus. R. E. Krieger Publishing Company, Huntington, New Yourk
  36. Refai, M. K. 1988. Aflatoxins and Aflatoxicosis. J. Egypt. Vet. Med. Ass. 48(1): 1-19
  37. Scott, P. M., Trenholm, H. L. and Sutton M. D. 1985. Mycotoxins: A canadian preservative publication, NRCC/CNRC, Ottawa, 185 pp
  38. Trojanowska, K. 1991. Evaluation of cereal grain quality using mycological methods. In: Developments in food science 26. Cereal grain: mycotoxins, fungi and quality in drying and storage. J. Chelkowski, ed. Elsevier, Amsterdam: pp. 185-215
  39. Tseng, T. C., Tu, J. C. and Tzean, S. S. 1995. Mycoflora and Mycotoxins in dry bean (Phaseolus vulgaris) produced in Taiwan and Ontario, Canada. Bot. Boll. Acad. Sin. 36: 229-234
  40. Van Egmond, H. P. 1981. Determination of mycotoxin. In J. F. Lawrence (ed.), Trace Analysis, vol. 1. New York Academic Press, pp. 99-144
  41. Wary, B. B. 1981. Aflatoxin, hepatitis, B. virus and hepatocellular carcinoma. New England J. Med. 305(14): 833-843
  42. Williams, R. J. and Rao, K. N. 1981. A review of sorghum grain moulds. Tropical Pest Management 27: 200-211 https://doi.org/10.1080/09670878109413652

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