Clinical and Experimental Reproductive Medicine

  • 제30권1호
  • /
  • Pages.65-75
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  • 2003
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

인간자궁내막의 탈락막화 (Decudualization)에 있어서 TGF-$\beta$ (Transforming Growth Factor-$\beta$)의 역할

Transforming Growth Factor-$\beta$ is a Possible Paracrine Mediator in the Human Endometrial Decidualization

  • 박동욱 (아주대학교 대학원 분자과학기술학과) ;
  • 최동순 (아주대학교 대학원 분자과학기술학과) ;
  • 김미란 (아주대학교 의과대학 산부인과학교실) ;
  • 황경주 (아주대학교 의과대학 산부인과학교실) ;
  • 조미영 (아주대학교 의과대학 산부인과학교실) ;
  • 안성희 (아주대학교 의과대학 산부인과학교실) ;
  • 민철기 (아주대학교 자연대학 생명과학과) ;
  • 유희석 (아주대학교 의과대학 산부인과학교실)
  • Park, Dong-Wook (Department Molecular Science and Technology, Ajou University) ;
  • Choi, Dong-Soon (Department Molecular Science and Technology, Ajou University) ;
  • Kim, Mi-Ran (Department of Obstetrics and Gynecology, School of Medicine, Ajou University) ;
  • Hwang, Kyung-Joo (Department of Obstetrics and Gynecology, School of Medicine, Ajou University) ;
  • Jo, Mi-Yeong (Department of Obstetrics and Gynecology, School of Medicine, Ajou University) ;
  • Ahn, Seong-Hee (Department of Obstetrics and Gynecology, School of Medicine, Ajou University) ;
  • Min, Churl-K. (Department of Biological Science, Ajou University) ;
  • Ryu, Hee-Sug (Department of Obstetrics and Gynecology, School of Medicine, Ajou University)
  • 발행 : 2003.03.30
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초록

Objectives: To investigate the role of TGF (Transforming growth factor-$\beta$) involved in the paracrinic communication during decidualization between UEC (uterine epithelial cells) and USC (uterine stromal cells), we have employed a co-culture system composed of human endometrial epithelial and stromal cells in defined hormonal conditions. Design: In the co-culture, endometrial epithelial cells cultured in the matrigel-coated cell culture insert are seeded on top of the endometrial stromal cells cultured within a collagen gel. The co-culture was maintained for 48 hours under the following hormonal conditions: progesterone dominant condition (100 nM P4 and 1 nM E2) or estrogen-dominant condition (100 nM E2 and 1 nM P4). 10 ng/ ml HGF and/or 10 ng/ml TGF-$\beta$1 are added. Methods: RT-PCR is utilized to detect mRNAs quantitatively. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining are utilized to detect proteins in the tissue. Results: Prolactin mRNA is expressed in the co-cultured stromal cells under the progesterone dominant condition. TGF-$\beta$1 and its receptors are expressed in both the co-cultured epithelial and stromal cells irrespective of the steroid present, which is in contrast with no or negligible expression of TGF-$\beta$1 or its receptor in cells separately cultured. Both estrogen and progesterone significantly elevate the concentration of hepatocyte growth factor (HGF) in the conditioned medium of the co-culture with the value of 4, 325 pg/ml in E2-dominant and 2, 000 pg/ml in P4-dominant condition compare to 150 pg/ml in no hormone. In separately cultured stromal cells, administration of HGF induces the expression of TGF receptor 1 in both hormonal conditions, but induction of TGF receptor 2 is only manifest in the P4-dominant condition. Administration of TGF-$\beta$ and HGF directly induce the decidualization marker prolactin mRNA in separately cultured stromal cells. Conclusion: It is likely that steroid hormones induces prolactin mRNA indirectly by promoting the cell to cell communication between the stromal and the epithelial cells. TGF-$\beta$ and HGF are two possible paracrine mediators in the human endometrial decidualization.

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