Clinical and Experimental Reproductive Medicine

  • 제30권1호
  • /
  • Pages.77-84
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  • 2003
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

착상전 생쥐 배아 발달에 대한 Apoptotic Gene의 발현

Expression of Apoptotic Genes in Mouse Preimplantation Embryo Development

  • 이여일 (전남대학교 의과대학 산부인과학교실) ;
  • 이진 (전남대학교 의과대학 산부인과학교실) ;
  • 김미영 (전남대학교 의과대학 산부인과학교실) ;
  • 전상영 (전남대학교 의과대학 호르몬연구센터)
  • Lee, Yu-Il (Department of Obstetrics and Gynecology, College of Medicine, Chonnam National University) ;
  • Lee, Jin (Department of Obstetrics and Gynecology, College of Medicine, Chonnam National University) ;
  • Kim, Mi-Young (Department of Obstetrics and Gynecology, College of Medicine, Chonnam National University) ;
  • Chun, Sang-Young (Hormone Research Center, Chonnam National University)
  • 발행 : 2003.03.30
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초록

Objective : The aim of this study was to evaluate the influence of three different media on preimplatation embryo development and the expression of Bcl-2, Mcl-1, Bax, and Bok in mouse. Materials and Methods: Two-cell embryos were retrieved from ICR female mice (4 weeks old) at 48 hr after hCG injection and cultured in Ham's F-10, HTF, and G1.2 media. The developmental rate of 2-cell embryos was evaluated from 24 hr to 72 hr after culture. RT-PCR was performed for the detection of Bcl-2, Mcl-1, Bax, and Bok gene expression. Results: The rates of morula and blastocyst in HTF and G1.2 media (88%, 98.1%) were significantly higher than those in Ham's F-10 media (39.6%) at 48 hr. Likewise, the rates of hatching and hatched blastocyst in HTF and G1.2 media (21.9%, 52.9%) were higher than those in Ham's F-10 media (3.5%) at 72 hr. Bcl-2 and Bax mRNAs were highly detected in embryos cultured in Ham's F-10 when compared in embryos cultured in HTF and G1.2. In contrast, the expression of Mcl-1 and Bok was not significantly different. Conclusion: These results show that HTF and G1.2 culture media increase the rate of blastocyst formation and stimulate Bcl-2 and Bax gene expression in mouse preimplantation embryos.

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