The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Inhibitory Effect of Achyranthes bidentata radix Extracts on Cytochrome P450-Catalyzed Reactions in Human Liver Microsomes

인체 간 Microsome에서 우슬 추출물의 Cytochrome P450 약물 대사효소에 대한 억제작용

  • 김경아 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 이지숙 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 박히준 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 김진우 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 김창주 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 심인섭 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 한승무 (경희대학교 동서의학연구소 통증 및 신경의학연구팀) ;
  • 임사비나 (경희대학교 동서의학연구소 통증 및 신경의학연구팀)
  • Published : 2003.06.01
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Abstract

Objectives : Achyranthes bidentata radix (Usul) has been used as anti-arthritic, antiallergic, antidiuretic, and so on. Recently extracts of Achyranthes bidentata radix have shown anti-inflammatory and cancer preventive effects in vitro and in vivo. Methods : We therefore evaluated the inhibitory potential of ethanol extracts of Achyranthes bidentata radix on cytochrome P450 (CYP) isoforms-catalyzed reactions, which relate to causes of cancer and inflammation, including CYP1A2, CYP2C9, CYP2C19, CYP2E1, CYP2D6, CYP2C8, and CYP3A4, using human liver microsomal preparations. Results : The extracts showed weak or negligible inhibitory effects on CYP2C9-catalyzed (S)-warfarin 7-hydroxylation, CYP2C19-catalyzed S-mephenytoin 4-hydroxylation, and CYP2D6-catalyzed dextromethorphan O-demethylation with each IC50 over 1750 g/ml, respectively. However, it showed relatively significant inhibitory effect on CYP1A2-catalyzed phenacetin O-deethylation and CYP2E1-catalyzed chlorzoxazone 6-hydroxylation with IC50s of 970.5 g/ml and 821.4 g/ml, respectively. Conclusions : These results suggest that extracts of Achyranthes bidentata radix have inhibitory effects on CYP-catalyzed reactions, especiallyCYP1A2 and CYP2E1, in human liver microsomes. These effects appear to relate to anti-inflammatory and cancer prevention following decrease of reactive oxygen species formed by CYP, especially CYP1A2 and CYP2E1, by Achyranthes bidentata radix. However, further evaluation is necessary to demonstrate and to confirm its effects in human.

Keywords

References

  1. Indian Forester v.96 Seasonal variation in chemical composition of Achyranrhes aspera and A. bidentata Ratra PS;Misra KC
  2. J Herbs Spices Med Plants v.2 Antinake venom botanicals from Ethnomedichine Selvanayagam ZE;Gnanavendan SG;Balakrishnan K;Rao RB
  3. Fitoterapia v.64 Constituents of Achyranrhes bidentata Bishit G;Sandhu H;Verma S
  4. J Chin Med. v.8 Studies on the chemical constituents of Achyranrhes bidentata Blume Chang YS;Chen CY;Ko LK
  5. Yao Xue Xue Bao. v.32 no.12 The immunomodulatory effect of Achyranthes bidentata polysaccharides Li ZK;Li DD
  6. Indian Journal of Pharmacology v.33 Bioflavonoids Classification, Pharmacological. Biochemical effects and Therapeutic potential Rajnarayana K;Sripal RM;Chaluvadi MR
  7. Phytotherapy Research v.11 Further study of anti-inflammatory effects of Abies pindrow Singh RK;Pandey BL
  8. J Nat Prod. v.64 no.2 Bidentatoside I, a new triterpene saponin from Achyranthes bidentata Mitaine-Offer AC;Marouf A;Pizza C;Khanh TC;Chauffert B;Lacaille-Dubois MA
  9. Lancet v.355 no.9198 Herb-drug interactions Fugh-Berman A.
  10. J Pharmacol Exp Ther. v.265 Specificity of substrate and inhibitor probes for human cytochrome P450 1A1 and 1A2 Tassaneeyakul W;Birkett DJ;Veronese ME;McManus ME;Turkey RH;Quattrochi LC;Gelboin HV;Miners JO
  11. Eur J Drug Metab Pharmacokinet v.23 no.3 Human liver microsomal metabolism of paclitaxel and drug interactions Desai PB;Duan JZ;Zhu YW;Kouzi S
  12. J Pharmacol Exp Ther. v.252 Tolbutamide and mephenytoin hydroxylation by human cytochrome P450s in the CYP2C subfamily Relling MV;Aoyama T;Gonzalez FJ;Meyer UA
  13. Arch Biochem Biophys. v.306 Isolation and characterization of human liver cytochrome P450 2C19: correlation between 2C19 and S-mephenytoin 4'-hydroxylation Wrighton SA;Stevens JC;Becker GW;Vanden Branden M
  14. Biopharm Drug Dispos. v.18 no.3 Biotransformation of chlorzoxazone by hepatic microsomes from humans and ten other mammalian species Court MH;Von Moltke LL;Shader RI;Greenblatt DJ
  15. J Pharmacol Exp Ther. v.276 Triazolam biotransformation by human liver microsomes in vitro: effects of metabolic inhibitors and clinical confirmation of a predicted interaction with ketoconazole Von Moltke LL;Greenblatt DJ;Harmatz JS;Duan SX;Harrel LM;Cotreau-Bibbo MM; Pritchard GA;Wright CE;Shader RI
  16. Br J Clin Pharmacol. v.49 In vitro inhibition of the cytochrome P450 (CYP450) system by the antiplatelet drug ticlopidine: potent effect on CYP2C19 and CYP2D6 Ko JW;Desta Z;Soukhova NV;Tracy T;Flockhart DA
  17. Herbalgram. v.44 The blooming U.S. botanical market: a new overview BrevootP
  18. Nat Biotechnol. v.17 Billion-dollar market blossoms as botanicals take root Glaser
  19. Tap Chi Duoc Hoc. v.6 Chemical research of the aerial part of Achyranrhes bidentata Blume Nguyen T;Nikalov S;Nguyen TD
  20. Pharmacol Rev. v.40 The molecular biology of cytochrome P450s Gonzalez FJ.
  21. Principles and Methods of Toxicology Analysis and characterization of enzymes Guengerich FP.;Hayes, A.W.(Ed.)
  22. J Free Radic Biol Med. v.1 no.1 Pathological mechanisms in carbon tetrachloride hepatotoxicity Brattin WJ;Glende EA Jr;Recknagel RO
  23. Pharmacol Ther. v.43 no.1 Mechanisms of carbon tetrachloride toxicity Recknagel RO;Glende EA Jr;Dolak JA;Waller RL
  24. Semin Liver Dis. v.10 no.4 Carbon tetrachloride hepatotoxicity: an example of free radical-mediated injury Williams AT;Burk RF
  25. Indian Journal of Pharmacology v.34 Effect of alcoholic extraction of Achyranrhes bidentata blume on acute and sub acute inflammation Vetrichelvan T;Jegadeesan M
  26. Free Radic Biol Med. v.28 no.10 Reactive oxygen species, cell signaling, and cell injury Hensley K;Robinson KA;Gabbita SP;Salsman S;Floyd RA